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Photo Credit: Jason Kilgore, Molecular Probes

Did you know: Emitted light is largely independent of the excitation wavelength.   Read More

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Introduction to Fluorescence

New to Chroma, microscopy, or fluorescence? Start here!

This section aims to introduce fluorescence by providing an understanding of the basic physics behind this natural phenomenon. Whether you are new to the field or have been using fluorescence for years, this section is a great overview of the science behind your work. Get started by navigating the links to the left and as always, if you have any further questions feel free to contact our support team!

 

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Brightness of Fluorescence Signal

Quantum efficiency getting quenched? We can help. 

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Fluorochrome Spectra

Thank you, Mr. Stokes.

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Percent Transmission and Optical Density

At Chroma, raising your transmission won't keep you from bottoming out...

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The Fluorescence Phenomenon

"Oh, Oh it's magic, ya know!" 

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Transmission, Reflection, and Absorption

If you could be any type of attenuated light you wanted, would you be reflected...or absorbed?

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Working in Wavelengths

Pick up an optical dictionary and you'll find that 'wavelength' is the first word.