This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke's shift.
**Prices are for unmounted filter sets. For the appropriate filter cubes/holders, please see our 91000 Series or contact us.**
The emission at these wavelengths will be better detected by camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths.
The emission at these wavelengths will be better detected by camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths.
This set will, in some cases, increase the signal-to-noise ratio of GFP imaged in live cells with high levels of auto-fluorescence. It will decrease the GFP signal substantially and should not be used with dim samples.
The emission at these wavelengths will be better detected by camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths.
This set is designed to image YFP. It will not, in most cases, detect CFP when co-expressed in cells. By eye, fluorescence in the 535 nm passband will be seen as green.
This longpass set was originally spec'd by the Tsien lab for visualization of coumarin and fluorescein in Beta-Lactamase assays. It is not designed for ratiometric imaging.
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