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Compare filter sets by selecting the recommended/alternative set listed for each fluorochrome below.

A B C D E F G H I J K L M N O P Q R S T W X Z
Recommended

WideField Sets

Recommended

Laser Sets

Fluor EX(nm) EM(nm)        
A
 
 
 
 
IF workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded. 49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.
   
39009 may be used with LEDs with CWL 470-490nm; 49024 may be used with 430-450nm CWL LEDs.
   
49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
   
49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 485-515nm CWL LEDs.
   
49014 may be used with LEDs with CWL520-540nm.
   
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
49008, 39010 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550 CWL LEDs.
   
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
49015 may be used with LEDs with CWL 585-620nm.
   
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
49022 may be used with LEDs with CWL 635-665nm.
   
49022 may be used with LEDs with CWL 635-665nm.
   
49007 may be used with LEDs with CWL 685-740nm.
   
49037 may be used with LEDs with CWL 725-755nm.
   
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.
   
AmCyan 458 489
49001, 39001 may be used with LEDs with CWL 430-440nm.
   
AsRed2 576 592
49005, 49305 may be used with LEDs with CWL 535-555nm.
   
Atto 390 390 479
49028 may be used with LEDs with CWL385-405nm.
   
Atto 425 436 485
49013 may be used with LEDs with CWL 435-455nm; 39001 may be used with 430-440nm CWL LEDs.
   
Atto 465 453 507
49002 may be used with LEDs with CWL 455-485nm.
   
Atto 488 501 525
49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
   
Atto 550 554 576
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
Atto 633 630 657
49015 may be used with LEDs with CWL 585-620nm.
   
Atto 647N 644 669
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
Atto 680 680 700
49022 may be used with LEDs with CWL 635-665nm.
   
Auramine 450 500
19008 may be used with LEDs with CWL 435-470nm.
   
Auramine-rhodamine 450 500-600
19002 may be used with LEDs with CWL 470-490nm.
   
Azami Green 492 505
49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
B
 
 
 
 
BCECF/pH 5.2 482 520
   
BCECF/pH 9.0 503 528
   
Biosearch Blue 352 447
49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs and 49028 may be used with 385-400nm CWL LEDs.
   
BODIPY FL/pH7.2 505 512
49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
   
C
 
 
 
 
49023 may be use with LEDs with CWL 490-520nm; 49014 may be used with 520-540nm CWL LEDs.
   
49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 490-520nm CWL LEDs.
   
49005 may be used with LEDs with CWL 535-555nm; 49004 may be used with 535-555nm CWL LEDs and 39004 may be used with 535-550nm CWL LEDs
   
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
49015 may be used with LEDs with CWL 585-620nm.
   
Calcein 494 517
49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
   
49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
   
Calcofluor White 350 450-500
Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 19012, 19000, and 19011 may be used successfully depending on the sample and the observer's preference. 19000 and 19012 may be used with LEDs with CWL 365-380nm; 19011 may be used with 395-410nm CWL LEDs.
   
Cerulean 433 475
49001, 39001 may be used with LEDs with CWL 430-440nm.
   
CFP 433 475
49001, 39001 may be used with LEDs with CWL 430-440nm.
   
Citrine 514 529
49003, 39003 may be used with LEDs with CWL 490-505nm.
   
Coumarin 384 470
49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 19011 may be used with 395-410nm CWL LEDs.
   
Cy2™ 489 506
49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
   
Cy3.5™ 581 598
49008, 39010 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550 CWL LEDs.
   
Cy3™ 552 570
49004, 49005 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
Cy5.5™ 675 695
49022 may be used with LEDs with CWL 635-665nm.
   
Cy5™ 649 670
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
Cy7™ 743 767
49007 may be used with LEDs with CWL 685-740nm.
   
D
 
 
 
 
DAPI 359 461
49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.
   
39009 may be used with LEDs with CWL 470-490nm.
   
This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.
   
DiA 456 591
49024 may be used with LEDs with CWL 425-455nm; 39009, 19010 may be used with 470-490nm CWL LEDs.
   
DiD 644 665
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.
   
DiI 551 569
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
DiO 484 502
49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
DiR 748 780
49007 may be used with LEDs with CWL 685-740nm.
   
Draq5 647 683
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.
   
DsRed 557 584
49004 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
DyLight 350 352 435
49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
   
DyLight 405 399 433
49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.
   
DyLight 488 492 517
49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
DyLight 549 554 569
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
DyLight 594 592 616
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
DyLight 633 623 645
49015 may be used with LEDs with CWL 585-620nm.
   
DyLight 649 652 667
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
DyLight 680 677 705
49022 may be used with LEDs with CWL 635-665nm.
   
DyLight 750 751 772
49007 may be used with LEDs with CWL 685-740nm.
   
DyLight 800 770 795
49037 may be used with LEDs with CWL 725-755nm.
   
E
 
 
 
 
EBFP2 385 448
49028 may be used with LEDs with CWL 385-400nm, 49021 may be used with 400-410nm CWL LEDs.
   
ECFP 434 477
49001, 39001 may be used with LEDs with CWL 430-440nm.
   
EGFP 488 507
49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
Emerald GFP 489 510
49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
Eosin 524 545
49023 may be used with LEDs with CWL 490-520nm.
   
49005 may be used with LEDs with CWL 535-555nm; 39009 may be used with 470-490nm CWL LEDs.
   
49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.
   
EYFP/pH 7 514 527
49003, 39003 may be used with LEDs with CWL 490-505nm.
   
F
 
 
 
 
FAM 492 518
49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
FITC 490 525
49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
FlAsH-CCPFCC 511 530
49003, 39003 may be used with LEDs with CWL 490-505nm.
   
Fluo-3 505 526
49003 may be used with LEDs with CWL 490-505nm; 49011 may be used with 465-495nm CWL LEDs.
   
Fluo-4 494 516
49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
FluoroGold 330-390 450-600
This dye has mainly been used as a retrograde neuronal tracer, although it can also distinguish between DNA and RNA binding according to its fluorescence emission. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI longpass filter sets 49025 and 19012 are appropriate for detection of the fluorescence emission associated with the both peaks, which together appear "gold". The 49025 set is also useful for distinguishing between FluoroGold bound to DNA or RNA.
   
FM™ 1-43 479 598
39009, 19010 may be used with LEDs with CWL 470-490nm.
   
FM™ 4-64 515 740
For imaging applications using a monochrome camera, this longpass filter set will not distinguish FM 4-64 from TRITC or similar orange/red fluorochromes. To be able to distinguish between FM 4-64 and TRITC or Texas Red-like fluorochromes, contact us for a custom filter set. 19010 may be used with LEDs with CWL 470-490nm; 19006 may be used with 545-575nm CWL LEDs.
   
   
   
FusionRed 576 603
49008, 39010 and 49017 may be used with LEDs with CWL 545-575nm.
   
G
 
 
 
 
GFP 488 507
EGFP spectra used here; similar to Emerald GFP, TagGFP, Azami Green, mWasabi, etc.49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
H
 
 
 
 
HEX,SE 533 559
49014 may be used with LEDs with CWL520-540nm; 49023 may be use with 485-515nm CWL LEDs.
   
Hoechst 33258 352 461
49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
   
I
 
 
 
 
79006 may be used with LEDs with CWL 365nm.
   
79006 may be used with LEDs with CWL 365nm.
   
The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties. 49030 may be used with LEDs with CWL 755-795nm.
   
J
 
 
 
 
JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously. 49012 may be used with LEDs with CWL 465-495nm; 19002 may be used with 470-490nm CWL LEDs.
   
JOE 520 548
49023 may be use with LEDs with CWL 485-515nm.
   
K
 
 
 
 
Killer Red 585 611
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
L
 
 
 
 
49022 may be used with LEDs with CWL 635-665nm.
   
49037 may be used with LEDs with CWL 725-755nm.
   
Lucifer Yellow 428 540
49026 may be used with LEDs with CWL 390-415nm; 39008 may be used with 405-430nm CWL LEDs.
   
49028 may be used with LEDs with CWL 385-400nm; 49000 may be used with LEDs with CWL 350-365nm; 39000 may be used with 365-380nm CWL LEDs.
   
49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
49008 may be used with LEDs with CWL 545-575nm; 49305 may be used with 535-550nm CWL LEDs.
   
49023 may be used with LEDs with CWL 485-515nm; 39009 may be use with 470-490nm CWL LEDs.
   
M
 
 
 
 
mCherry 587 610
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
mCitrine 514 529
49003, 39003 may be used with LEDs with CWL 490-505nm; 49023 may be use with 490-520nm CWL LEDs.
   
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
49002 may be used with LEDs with CWL 455-485nm; 49011 may be used with 465-495nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
mKate2 589 634
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
mKeima Red 440 620
49024 may be used with LEDs with CWL 430-450nm.
   
mKO 548 559
49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.
   
mOrange2 548 562
49014 may be used with LEDs with CWL 520-540nm; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.
   
mPlum 589 648
49017, 19006 may be used with LEDs with CWL 545-575nm
   
mRFP1 584 607
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
mTFP1 462 492
49013 may be used with LEDs with CWL 435-455nm.
   
mWasabi 493 509
49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
N
 
 
 
 
NBD X/MeOH 467 538
49011 may be used with LEDs with CWL 465-495nm; 19008 may be used with 435-465nm CWL LEDs.
   
Nile Blue 631 660
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs.
   
NirFP 604 669
49019 may be used with LEDs with CWL 595-640nm.
   
O
 
 
 
 
49011, 39002 may be used with LEDs with CWL 470-490nm; 49003 set may be used with 490-505nm LED.
   
49003, 39003 may be used with LEDs with CWL 490-505nm. 49303 may be used with 490-505nm CWL LEDs.
   
P
 
 
 
 
Pacific Blue 405 455
49021 may be used with LEDs with CWL 400-410nm; 19011 may be used with 395-410nm CWL LEDs.
   
49005 may be used with LEDs with CWL 535-555nm; 39005 may be used with 535-550nm CWL LEDs.
   
Pulsar™ 650 460 650
49024 may be used with LEDs with CWL 430-450nm; 39009, 19010 may be used with 470-490nm CWL LEDs.
   
Q
 
 
 
 
Qdot 525 250-450 525
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 545 250-450 545
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 565 250-450 565
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 585 250-450 585
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 605 250-450 605
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 625 250-450 625
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 655 250-450 655
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 705 250-450 705
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Qdot 800 250-450 800
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
   
Quasar® 570 548 566
49014 may be used with LEDs with CWL 520-540nm; 49004 may be used with 535-555nm CWL LEDs.
   
Quasar® 670 647 670
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
Quasar® 705 690 705
49022 may be used with LEDs with CWL 635-670nm.
   
R
 
 
 
 
R-phycoerythrin 565 578
49014 may be used with LEDs with CWL 520-540nm; 19004 may be used with 535-545nm CWL LEDs; 49010 is not appropriate for use with LEDs because the bandwidth is too narrow.
   
ReAsH-CCPGCC 592 606
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
Rhod-2 552 581
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
Rhodamine 123 507 529
49003, 39003 may be used with LEDs with CWL 490-505nm.
   
Rhodamine 6G 525 555
49023 may be use with LEDs with CWL 485-515nm.
   
49008, 39010 may be used with LEDs with CWL 545-575nm.
   
ROX 578 604
49008, 39010 may be used with LEDs with CWL 545-575nm.
   
S
 
 
 
 
SBFI/Na+ -free 340 505
   
   
49008, 39010 may be used with LEDs with CWL 545-575nm.
   
SYBR® Green I 498 522
49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
SYTO 9/DNA 483 500
49011, 39002 may be used with LEDs with CWL 465-495nm; 49002 may be used with 455-485nm CWL LEDs.
   
SYTO® 60 652 678
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
T
 
 
 
 
TagBFP 402 457
49021 may be used with 400-410nm CWL LEDs, 49028 may be used with 385-400nm CWL LEDs.
   
TagRFP 556 584
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
TAMRA 555 580
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
tdTomato 554 581
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
TET 521 536
49023 may be used with LEDs with CWL 485-515nm.
   
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
Texas Red® 595 620
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
Texas Red®-X 595 615
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
TO-PRO™-3 642 661
49006 may be used with LEDs with CWL 595-640nm; 39007 may be used with 600-635nm CWL LEDs; 49009 may be used with 625-650nm CWL LEDs.
   
Topaz 514 529
49003, 39003 may be used with LEDs with CWL 490-505nm.
   
TRITC 555 580
49004, 49305 may be used with LEDs with CWL 535-555nm; 39004 may be used with 535-550nm CWL LEDs.
   
TurboFP650 592 650
49008, 49017 and 19006 may be used with LEDs with CWL 545-575nm.
   
W
 
 
 
 
wtGFP 475 509
19008 may be used with LEDs with CWL 435-470nm; 49002 may be used with 455-485nm CWL LEDs
   
X
 
 
 
 
X-rhod-1/Ca2+ 580 601
49008, 39010 may be used with LEDs with CWL 545-575nm; 49306 may be used with 570-595nm CWL LEDs.
   
Z
 
 
 
 
ZsGreen1 493 505
49002 may be used with LEDs with CWL 455-485nm; 49020 may be used with 475-485nm CWL LEDs; 39002 may be used with 470-490nm CWL LEDs.
   
ZsYellow 529 539
49023 may be use with LEDs with CWL 485-515nm; 49003 set may be used with 490-505nm LED.
   
 
 
 
 
   
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