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Chroma Technology Corp

Browse by Fluorochrome

Find filter sets by choosing the recommended or alternative set listed for each fluorochrome below.

1-9  A B C D E F G H I J L M N O P Q R S T W X Z
Fluor EX(nm) EM(nm) Available Sets
1-9
5-(and 6)-carboxy-2',7'-dichlorofluorescein 512 530
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
A
Acridine Orange + DNA 500 526
Recommended:ET - EGFP (FITC/Cy2)
Alternative:ET - FITC/EGFP Longpass
Alexa Fluor 350™ 346 442
Recommended:ET - DAPI
Alternative:DAPI/Hoechst/AMCA
Alexa Fluor 488™ 498 520
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
Alexa Fluor 488™ 498 520
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:FITC/ EGFP/ Bodipy Fl/ Fluo3/DiO
Alexa Fluor 514™ 517 539
Recommended:ET - EYFP
Alternative:EYFP/Venus/Citrine/Topaz Bandpass
Alexa Fluor 532™ 531 554
Contact us for custom filter set recommendations
Alexa Fluor 546™ 556 573
Recommended:ET - CY3/TRITC
Alternative:TRITC (Rhodamine)/DiI/Cy3™/AF546 with narrow-band excitation filter
Alexa Fluor 555™ 553 568
Recommended:ET - CY3/TRITC
Alternative:TRITC (Rhodamine)/DiI/Cy3™/AF546 with narrow-band excitation filter
Alexa Fluor 568™ 578 603
Recommended:ET - mCherry, Texas Red®
Alternative:Cy3.5™
Alexa Fluor 594™ 590 617
Recommended:ET - mCherry, Texas Red®
Alternative:Texas Red®
Alexa Fluor 610™
Contact us for custom filter set recommendations
Alexa Fluor 633™ 632 647
Recommended:ET - Cy5 Narrow Excitation
Alternative:Visual Cy5™ (GLF)
Alexa Fluor 647™ 649 666
Recommended:ET - Cy5
Alternative:ET - Cy5 Narrow Excitation
Alexa Fluor 700™ 696 719
Recommended:ET - Cy7
Alternative:Cy5.5™
Alexa Fluor 750™ 752 779
Recommended:ET - Cy7
Alternative:Cy7™
Alexa Fluor 790™ 782 805
Recommended:ET - Li_Cor for IR dye 800
Allophycocyanin (APC) 630 660
Recommended:ET - Cy5
Alternative:Cy5™
AmCyan 458 489
Recommended:ET - ECFP
AsRed2 576 592
Recommended:ET - DSRed (TRITC/Cy3)
Azami Green 492 505
Recommended:ET - EGFP (FITC/Cy2)
Alternative:FITC/ EGFP/ Bodipy Fl/ Fluo3/DiO
B
BCECF/pH 5.2 482 520
BCECF/pH 9.0 503 528
BODIPY FL/pH7.2 505 512
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
C
Calcein 494 517
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - FITC/EGFP Longpass
Calcium Green™-1 506 531
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
Calcofluor White 355 440
Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 11000, 11003, 11005, 11006, 11011 and 11012 may be used successfully depending on the sample and the observer’s preference.
Recommended:UV
Alternative:Violet
Carboxyrhodamine 6G 518 543
Recommended:Eosin/ZsYellow
Alternative:ET - FITC/EGFP Longpass
Cerulean 433 475
Recommended:ET - ECFP
CFP 433 475
Recommended:ET - ECFP
Coumarin 384 470
Cy2™ 489 506
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
Cy3.5™ 581 598
Recommended:Cy3.5™
Cy3™ 552 570
Recommended:ET - CY3/TRITC
Alternative:ET - DSRed (TRITC/Cy3)
Cy5.5™ 675 695
Recommended:Cy5.5™
Alternative:ET - Cy5
Cy5™ 649 670
Recommended:ET - Cy5
Alternative:ET - Cy5 Narrow Excitation
Cy7™ 743 767
Recommended:ET - Cy7
Alternative:ET - Cy5 Narrow Excitation
D
DAPI 359 461
Recommended:ET - DAPI
Di-8-ANEPPS non-ratiometric 530 620
Recommended:Di-8-ANEPPS (non ratio)
Di-8-ANEPPS, ratiometric
This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.
DiA 456 591
Recommended:DiA
DiD 644 665
Recommended:ET - Cy5
DiI 549 565
Recommended:ET - DSRed (TRITC/Cy3)
Alternative:ET - CY3/TRITC
DiO 484 502
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
DiR 748 780
Recommended:ET - Cy7
Alternative:Cy7™
Draq5 647 683
Recommended:ET - Cy5
Alternative:Cy5™
DsRed2 563 582
Recommended:ET - DSRed (TRITC/Cy3)
Alternative:ET - CY3/TRITC
DyLight 350 352 435
Recommended:ET - DAPI
Alternative:DAPI/Hoechst/AMCA
DyLight 405 399 433
Recommended:ET - EBFP2/Coumarin/Attenuated DAPI
Alternative:Hydroxycoumarin
DyLight 488 492 517
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:FITC/ EGFP/ Bodipy Fl/ Fluo3/DiO
DyLight 549 554 569
Recommended:ET - CY3/TRITC
Alternative:TRITC (Rhodamine)/DiI/Cy3™/AF546 with narrow-band excitation filter
DyLight 594 592 616
Recommended:ET - mCherry, Texas Red®
Alternative:Texas Red®
DyLight 633 623 645
Recommended:Visual Cy5™ (GLF)
Alternative:ET - Cy5
DyLight 649 652 667
Recommended:ET - Cy5
Alternative:Cy5™
DyLight 680 677 705
Recommended:Cy5.5™ (Red Shifted)
Alternative:Cy5.5™
DyLight 750 751 772
Recommended:ET - Li_Cor for IR dye 800
Alternative:Cy7™
DyLight 800 770 795
Recommended:ET - Li_Cor for IR dye 800
Alternative:Li-Cor for IR Dye 800
E
EBFP2 385 448
Recommended:ET - EBFP2/Coumarin/Attenuated DAPI
EGFP 488 507
Recommended:ET - EGFP (FITC/Cy2)
Alternative:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Emerald GFP 489 510
Recommended:ET - EGFP (FITC/Cy2)
Alternative:Endow GFP/EGFP Bandpass
Eosin 524 545
Recommended:Eosin/ZsYellow
Ethidium Bromide 510 595
Recommended:Cy3™/TRITC (Rhodamine)/DiI
Ethidium homidimer-1/DNA 527 617
Recommended:Cy3™/TRITC (Rhodamine)/DiI
EYFP/pH 7 514 527
Recommended:ET - EYFP
Alternative:EYFP/Venus/Citrine/Topaz Bandpass
F
FAM 492 518
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
FITC 490 525
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
FlAsH-CCPFCC 511 530
Recommended:ET - EYFP
Alternative:EYFP/Venus/Citrine/Topaz Bandpass
Fluo-3 505 526
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
Fluo-4 494 516
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
FluoroGold
This dye has mainly been used as a retrograde neuronal tracer. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI bandpass filter sets 49000 and 31000 are appropriate for detection of the fluorescence emission associated with the 450nm peak. A longpass set such as our basic UV set 11000 will also allow detection of the red wavelength emission, which together with the blue emission, appears “gold”.
FM™ 1-43 479 598
Contact us for custom filter set recommendations
FM™ 4-64 515 740
Contact us for custom filter set recommendations
Fura Red/Ca2+ - saturated 436 655
Recommended:Fura Red
Fura Red/Ca2+ -free 473 670
Recommended:Fura Red
Fura-2/Ca2+ - free 340 510
Recommended:ET FURA2
Fura-2/Ca2+ - saturated 380 510
Recommended:ET FURA2
G
GFP 488 507
Recommended:ET - EGFP (FITC/Cy2)
H
HEX,SE 533 559
Recommended:ET - R&B Phycoerythrin/mOrange/mKO
Alternative:Eosin/ZsYellow
Hoechst 33258 352 461
Recommended:ET - DAPI
I
Indo-1/Ca2+ -free 346 475
indo-1/Ca2+ -saturated 330 401
Indocyanine Green
The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties.
Recommended:ET - Indocyanine Green
J
JC-1 non-ratiometric
JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously.
Recommended:ET - FITC/EGFP Longpass
Alternative:FITC/ EGFP/ Bodipy Fl/ Fluo3/ DiO Longpass
JC-1 ratiometric
JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. Use of this 71019 filter set requires an external filter wheel to house the 2 emission filters to facilitate rapid switching of filters for ratiometric measurements.
Recommended:JC1
JOE 520 548
Recommended:Eosin/ZsYellow
L
Lucifer Yellow 428 540
Recommended:Lucifer Yellow
LysoTracker Blue/MeOH 373 425
Recommended:ET - DAPI
LysoTracker Green/pH 5.2 504 511
Recommended:ET - EGFP (FITC/Cy2)
LysoTracker Red/pH 5.2 577 590
Recommended:ET - mCherry, Texas Red®
LysoTracker Yellow HCK-123 465 535
Recommended:ET - CY3/TRITC
M
mCherry 587 610
Recommended:ET - mCherry, Texas Red®
mCitrine 514 529
Recommended:ET - EYFP
MitoTracker Deep Red 633/MeOH 644 665
Recommended:ET - mCherry, Texas Red®
MitoTracker Green FM/MeOH 490 516
Recommended:ET - EGFP (FITC/Cy2)
MitoTracker Orange/MeOH 551 576
Recommended:ET - DSRed (TRITC/Cy3)
mKate (TagFP635) 588 635
Recommended:ET - mCherry, Texas Red®
Alternative:HcRed1
mKO 548 559
Recommended:ET - R&B Phycoerythrin/mOrange/mKO
mOrange 548 562
Recommended:ET - R&B Phycoerythrin/mOrange/mKO
mRFP1 584 607
Recommended:ET - mCherry, Texas Red®
mTFP1 462 492
Contact us for custom filter set recommendations
mWasabi 493 509
Recommended:ET - EGFP (FITC/Cy2)
Alternative:Endow GFP/EGFP Bandpass
N
NBD X/MeOH 467 538
Recommended:NBD / HPTS
Nile Blue 631 660
Recommended:ET - Cy5
O
Oregon Green™ 488 490 514
Recommended:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Alternative:ET - EGFP (FITC/Cy2)
Oregon Green™ 514 506 526
Recommended:ET - EYFP
P
Pacific Blue 405 455
Recommended:ET - EBFP2/Coumarin/Attenuated DAPI
Alternative:Pacific Blue
Propidium Iodide 536 617
Recommended:Propidium Iodide
Q
Qdot 525 350 525
Recommended:Qdot 525 with 40nm emission filter
Qdot 545 350 545
Recommended:Catskill Green
Qdot 565 350 565
Recommended:Qdot 565 with 40nm emission filter
Qdot 585 350 585
Recommended:Qdot 585 with 40nm emission filter
Qdot 605 350 605
Recommended:Qdot 605 with 40nm emission filter
Qdot 625 streptavidin/pH 7.2 351 606
Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
Recommended:Maple Red Orange
Qdot 655 350 655
Recommended:Qdot 655 with 40nm emission filter
Qdot 705 350 705
Recommended:Qdot 705 filter set with 40nm wide emission
Qdot 800 350 800
Recommended:Qdot 800
R
R-phycoerythrin 565 578
Recommended:ET - R&B Phycoerythrin/mOrange/mKO
ReAsH-CCPGCC 592 606
Recommended:ET - mCherry, Texas Red®
Resorufin 571 585
Recommended:Resorufin
Alternative:ET - DSRed (TRITC/Cy3)
Rhod-2 540 576
Recommended:ET - CY3/TRITC
Alternative:ET - DSRed (TRITC/Cy3)
Rhodamine 123 507 529
Recommended:ET - EYFP
Alternative:Eosin/ZsYellow
Rhodamine 6G 525 555
Recommended:Rhodamine X / Resorufin
Rhodamine Red™-X 570 590
Recommended:ET - mCherry, Texas Red®
ROX 578 604
Recommended:ET - mCherry, Texas Red®
Alternative:Texas Red®
S
SBFI/Na+ -free 340 505
Recommended:ET FURA2
SBFI/Na+ -saturated 380 505
Recommended:ET FURA2
Sulforhodamine 101 586 605
Recommended:ET - mCherry, Texas Red®
SYBR® Green I 498 522
Recommended:ET - EGFP (FITC/Cy2)
Alternative:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
SYTO 9/DNA 483 500
Recommended:ET - EGFP (FITC/Cy2)
Alternative:ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
SYTO® 60 652 678
Recommended:ET - Cy5
T
T-Sapphire 398 510
Contact us for custom filter set recommendations
TagBFP 402 457
Recommended:ET - EBFP2/Coumarin/Attenuated DAPI
TagRFP 556 584
Recommended:ET - CY3/TRITC
Alternative:TRITC (Rhodamine)/DiI/Cy3™/AF546 with narrow-band excitation filter
TAMRA 555 580
Recommended:ET - CY3/TRITC
Alternative:ET - DSRed (TRITC/Cy3)
tdTomato 554 581
Recommended:ET - DSRed (TRITC/Cy3)
Alternative:ET - CY3/TRITC
TET 521 536
Recommended:Eosin/ZsYellow
Tetracycline 405 550
Tetramethylrhodamine goat anti–mouse IgG antibody/ 555 580
Recommended:ET - CY3/TRITC
Alternative:ET - DSRed (TRITC/Cy3)
Texas Red® 595 620
Recommended:ET - mCherry, Texas Red®
Texas Red®-X 595 615
Recommended:ET - mCherry, Texas Red®
TO-PRO™-3 642 661
Recommended:ET - Cy5
W
wtGFP 475 509
Recommended:ET - EGFP (FITC/Cy2)
X
X-rhod-1/Ca2+ 580 601
Recommended:ET - mCherry, Texas Red®
Z
ZsGreen1 493 505
Recommended:ET - EGFP (FITC/Cy2)
ZsYellow 529 539
Recommended:Eosin/ZsYellow