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Browse by Fluorochrome
Compare filter sets by selecting the recommended/alternative set listed for each fluorochrome below.
Recommended
WideField Sets
Recommended
Laser Sets
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IF workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded.
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B |
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C |
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Calcofluor White is a commonly used stain to detect fungi and yeast and other microorganisms. It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. Calcofluor White has extremely broad excitation/emission spectra, and as a result almost any filter set with UV or violet/blue excitation wavelengths will generate fluorescence emission which may be detected across a very broad range. Some samples will fluoresce strongly in the green wavelengths, others in the blue, and others yellow-orange. Because of this, filter selection is subjective, partly depending on the experience of the microscopist. Our filter sets 11000, 11003, 11005, 11006, 11011 and 11012 may be used successfully depending on the sample and the observer’s preference.
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D |
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This dye and the related dye, Di-4-ANEPPS, are most effectively used as ratiometric indicators of membrane potential. Increases in membrane potential are indicated by a decrease in fluorescence intensity with approx. 440nm excitation and an increase in fluorescence intensity with approx. 530nm excitation. Use of this 71006 filter set requires an external filter wheel to house the 2 excitation filters and to facilitate rapid switching of filters for ratiometric measurements.
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E |
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F |
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This dye has mainly been used as a retrograde neuronal tracer. With two excitation peaks at approx. 330nm and 390nm, and two emission peaks at approx. 450nm and 600nm, our DAPI bandpass filter sets 49000 and 31000 are appropriate for detection of the fluorescence emission associated with the 450nm peak. A longpass set such as our basic UV set 11000 will also allow detection of the red wavelength emission, which together with the blue emission, appears "gold".
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For imaging applications using a monochrome camera, this longpass filter set will not distinguish FM 4-64 from TRITC or similar orange/red fluorochromes. To be able to distinguish between FM 4-64 and TRITC or Texas Red-like fluorochromes, contact us for a custom filter set.
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G |
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EGFP spectra used here; similar to Emerald GFP, TagGFP, Azami Green, mWasabi, etc.
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H |
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I |
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The spectral properties of ICG are strongly dependent on its concentration and the solute it is bound to, so care must be taken by workers to characterize their particular model system. This filter set 49030 is appropriate for most applications involving protein-bound ICG in various physiological media. ICG becomes concentrated in lipid environments, greatly shifting its spectral properties.
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J |
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JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. The 49012 longpass filter set will allow detection of JC-1 monomer and aggregate simultaneously.
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JC-1 exists as a monomer at low concentrations and/or low membrane potentials and displays fluorescence ex./em. maxima similar to that of YFP. At higher concentrations and/or higher membrane potentials, JC-1 forms aggregates with a red-shifted emission maxima of 590nm and a broadened excitation spectra. Use of this 71019 filter set requires an external filter wheel to house the 2 emission filters to facilitate rapid switching of filters for ratiometric measurements.
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K |
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L |
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M |
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N |
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O |
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P |
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Q |
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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Although the excitation maxima of qdot nanocrystals is in the UV, a broad range of excitation wavelengths may be used.
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R |
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S |
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T |
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W |
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X |
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Z |
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