Browse by Series
49000 Series - ET Sputtered Single Band Filter Sets
This series features Chroma’s Modified Magnetron sputter coatings (ET) which achieve the highest transmission levels for interference filters. Like the 41000 series, these filters also have a very steep transition from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and will last much longer than soft-coatings. Additionally, use of our ET filters assures image registration between filter sets in imaging systems which are otherwise in perfect register.
49300 Series - ET Sputtered Single Band Filter Sets for FISH and Chromosome Painting
Chroma’s 49300 Series of filter sets are identical to the 49000 ET single band sets in design and construction, but are intended to be used with common, commercially available FISH probes and chromosome painting probes. These high-throughput ET filter sets are spectrally similar to sets commonly used to detect the family of Spectrum probes from Abbott Laboratories, and similar probes from Cytocell and other manufacturers of genetic assays. Like the 49000 Series, these sets are intended for use with non-laser light sources.
49900 Series - ET Sputtered Single Band Laser Filter Sets
Our Sputtered Single Band Laser filter sets employ the same high-transmission, high-durability coatings as our other ET sets. These sets reflect a single wavelength range and consist of longpass transmission dichroics, laser clean-up filters and laser emission filters. In addition, all laser sets feature our Ultra-Flat laser dichroics. Chroma has developed a proprietary manufacturing method which minimizes surface curvature, and all of our laser dichroics with 1mm thick substrates are <2 lambda/inch Peak-Valley surface flatness. For more demanding applications such as TIRF, STED, structured illumination or PALM/STORM, we recommend our thicker dichroics mounted into Chroma’s custom metal cubes. Our manufacturing process, coupled with thicker substrates, provides the highest level of surface flatness in the industry for imaging dichroics used in microscope cubes.
TRF49900 - ET Sputtered Single Band Laser TIRF Filter Sets
The TRF49900 single band TIRF filter sets utilize the same laser clean-up filter and bandpass emission filter as the corresponding 49900 series filter set, but also contain an additional longpass emission filter for greatly increased blocking of the TIRF laser. Both emission filters are mounted in the filter cube to provide Ultra-High signal/noise ratios. The Ultra-Flat Dichroics in our TIRF sets are 2mm thick for distortion-free reflection of TIRF lasers with surface flatness =/> 0.5 waves/inch P-V, RWD. All catalog TIRF sets are mounted in Chroma’s metal TIRF cubes. For more information, please see the supporting materials on our TIRF filter set webpages.
59000 Series - ET Sputtered Dual Band Filter Sets and Dual Band Sets With Single Band Exciters
This series of dual-band filter sets feature our high performance ET sputter coatings. Emission filters have two passbands specific for a particular pair of fluorochromes. The sets with dual-band excitation filters allow for simultaneous visualization of two fluorochromes as often required for applications such as FISH or screening assays. The sets with two single-band excitation filters allow for selective excitation, but require the use of excitation filter wheels or sliders. NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
TRF59900 - ET Sputtered Dual Band Laser TIRF Filter Sets
We offer two versions of each our TRF59900 dual band TIRF filter sets, each providing greater levels of blocking of the TIRF lasers than our 59900 series filter sets. One version (“-EM”) utilizes additional, single band emission filters which match the Dual band emission filter in the filter cube. This option requires an emission filter wheel to house the additional emission filters. The other option relies on one dual band emission filter which provides extra blocking of the TIRF laser, and is housed in the filter cube. The highest signal/noise levels are obtained with the “-EM” versions, however for workers who need very fast switching or who don’t have a filter wheel, the alternate version is an excellent option. The Ultra-Flat Dichroics in our TIRF sets are 2mm thick for distortion-free reflection of TIRF lasers with surface flatness =/> 0.5 waves/inch P-V, RWD. All catalog TIRF sets are mounted in Chroma’s metal TIRF cubes. For more information, please see the supporting materials on our TIRF filter set webpages.
59900 Series - ET Sputtered Dual Band Laser Filter Sets
Our Sputtered Dual Band Laser filter sets employ the same high-transmission, high-durability coatings as our other ET sets. These sets reflect two different laser wavelength ranges and consist of dual band transmission dichroics, dual band laser clean-up filters and dual band laser emission filters. In addition, all laser sets feature our Ultra-Flat laser dichroics. Chroma has developed a proprietary manufacturing method which minimizes surface curvature, and all of our laser dichroics with 1mm thick substrates are <2 lambda/inch peak-valley surface flatness. For more demanding applications such as TIRF, STED, structured illumination or PALM/STORM, we recommend our thicker dichroics mounted into Chroma’s custom metal cubes. Our manufacturing process, coupled with thicker substrates, provides the highest level of surface flatness in the industry for imaging dichroics used in microscope cubes.
69000 Series - ET Sputtered Triple Band Filter Sets and Triple Band Sets with Single Band Exciters
This series of triple-band filter sets feature our high performance ET sputter coatings. Emission filters have three passbands specific for a particular trio of fluorochromes. The sets with triple-band excitation filters allow for simultaneous visualization of three fluorochromes as often required for applications such as FISH or screening assays. The sets with three single-band excitation filters allow for selective excitation, but require the use of excitation filter wheels or sliders. NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
89000 Series - ET Sputtered Multi-Band Sets
This series of multi-band filter sets feature our high performance ET sputter coatings. Included are dual, triple and quad-band sets, all of which use single-band excitation and emission filters. These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters. The polychroic beamsplitters remain stationary in a filter cube or similar holder. These sets are image-registered and are particularly useful for co-localization studies, FRET, and simultaneous imaging in live cell applications using image-splitting devices.
89900 - ET Sputtered Quad Band Laser Filter Sets
Our Sputtered quad band laser filter sets employ the same high-transmission, high-durability coatings as our other ET sets. These sets reflect four different laser wavelength ranges and consist of quad band transmission dichroics, quad band laser clean-up filters and quad band laser emission filters. In addition, all laser sets feature our Ultra-Flat laser dichroics. Chroma has developed a proprietary manufacturing method which minimizes surface curvature, and all of our laser dichroics with 1mm thick substrates are <2 lambda/inch Peak-Valley surface flatness. For more demanding applications such as TIRF, STED, structured illumination or PALM/STORM, we recommend our thicker dichroics mounted into Chroma’s custom metal cubes. Our manufacturing process, coupled with thicker substrates, provides the highest level of surface flatness in the industry for imaging dichroics used in microscope cubes.
TRF89900 - ET Sputtered Quad Band Laser TIRF Filter Sets
We offer two versions of each our TRF89900 dual band TIRF filter sets, each providing greater levels of blocking of the TIRF lasers than our 89900 series filter sets. One version (“-EM”) utilizes additional, single band emission filters which match the quad band emission filter in the filter cube. This option requires an emission filter wheel to house the additional emission filters. The other option relies on one quad band emission filter which provides extra blocking of the TIRF laser, and is housed in the filter cube. The highest signal/noise levels are obtained with the “-EM” versions, however for workers who need very fast switching or who don’t have a filter wheel, the alternate version is an excellent option. The Ultra-Flat Dichroics in our TIRF sets are 2mm thick for distortion-free reflection of TIRF lasers with surface flatness =/> 0.5 waves/inch P-V, RWD. All catalog TIRF sets are mounted in Chroma’s metal TIRF cubes. For more information, please see the supporting materials on our TIRF filter set webpages.
91000 Series - Filter Holders (cubes)
We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us.
41000 Series - HQ Filter Sets
This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke's shift.
43000 Series - BioPixels Filter Sets
This series contains sets optimized for use with BioPixels functionalized quantum dot nanoparticles. Sets are available to resolve nanocrystals individually using bandpass emission filters or in concert using a longpass emission filter.
51000 Series - Dual Band Filter Sets
The dual-band filter sets were designed for simultaneous viewing of two fluorochromes. Each excitation filter contains two passbands for simultaneous excitation. Concurrently, each emission filter contains two passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. These sets were originally designed for FISH (fluorescence in situ hybridization). During their history, their performance has been relied upon in many other applications.
61000 Series - Triple Band Filter Sets
The triple band filter sets were designed for simultaneous viewing of three fluorochromes. Each excitation filter contains three passbands for simultaneous excitation. Concurrently each emission filter contains three passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. Like the dual band series, these sets were originally designed for FISH (fluorescence in situ hybridization). Also like the dual band sets these filter sets have shown great practicality for other applications.
70000 Series - Dual Excitation and Dual Emission Dye Filter Sets
This series of filters was designed to allow either multiple excitation or multiple emission of a fluorochrome. By using filters mounted in either a wheel or slider, this design allows for ratiometry utilizing either two excitation wavelengths or two emissions. This capability obviously requires specialized hardware and software on the part of the investigator.
MF100 - Filter Sets for Multi-Color FISH (Spec'd by Perceptive Scientific for Vysis fluorochromes)
Specified by Perceptive Scientific for use with Vysis fluorochromes. Please contact Vysis for the official Vysis M-FISH filter sets.
SP100 - Filter Sets for Multi-Color FISH
Designed for Dr. Michael Speicher, University of Munich. Each of the components of these filter sets (except for the SP100 set) are even steeper than the 41 series. This allows for color discrimination when using up to seven different fluorochromes. To increase brightness, each of the components is anti-reflected. Again, the SP100 is the exception.
11000 Series - Basic Filter Sets
The most fundamental of Chroma's filter sets. These sets were designed primarily for viewing the fluorescence of single fluorochrome. All of these sets contain longpass emission filters. Longpass emission filters cannot exclude the fluorescence of a second fluorochrome which may also be excited. For instance, blue light, which is the primary excitation color for FITC, will also excite, albeit slightly less efficiently, than TRITC. A longpass emission filter will be unable to exclude the fluorescence of TRITC. Most of the barrier filters of our Basic sets are made from absorptive glass.
31000 Series - Standard Filter Sets
By replacing the longpass emission filter of the 11/Basic Series with bandpass emission filters Chroma's Standard series prevents bleed-through of undesired long wavelength fluorescence: for example, preventing red fluorescence from appearing in the green image). These filters also tend to decrease the problems associated with autofluorescence.
32000 Series - QDot - Wide Excitation
This series contains the original filter sets designed for Quantum Dot Corp. These sets are specifically developed with wide excitation to capture all of the energy from UV to blue. Note: this wide excitation may not be necessary for all applications, and could result in increased autofluorescence.
32200 Series - EviTag - Wide Excitation
This series contains the original filter sets designed for Evident Technologies. These sets are specifically developed with wide excitation to capture all of the energy from UV to blue. Note: this wide excitation may not be necessary for all applications, and could result in increased autofluorescence.
35000 Series - Live/Dead Filter Sets
Live/Dead sets for determining cytotoxicity using Molecular Probes Reagents.
80000 Series - Multi-Band Filter Sets with Single Emitters and Multiple Exciters
Originally designed for Comparative Genomic Hybridization the filter sets in this series, like those in the 51 and 61 series have become important in a wide variety of applications. The factors common to each of the 80 series filter sets is the use of a multiband beamsplitter and emission filter combination which remain stable in the body of the fluorescence microscope. The use of a stable beamsplitter and emission filter guarantees registration of the various images. The single, and sometimes multi-band excitation filters are mounted in a filter wheel or a filter slider. By changing excitation filters one seeks to excite each fluorochrome in succession, capturing a single image and combining those images with the help of imaging software. There can be some bleeding of one fluorochrome into the image of a shorter wavelength fluorochrome. This is sometimes inevitable, as is the case with Propidium Iodide and FITC.
88000 Series - Quad Filter Set with Single Band Exciters
Originally designed for Comparative Genomic Hybridization the filter sets in this series, like those in the 51 and 61 series have become important in a wide variety of applications. The factors common to each of the 80 series filter sets is the use of a multiband beamsplitter and emission filter combination which remain stable in the body of the fluorescence microscope. The use of a stable beamsplitter and emission filter guarantees registration of the various images. The single, and sometimes multi-band excitation filters are mounted in a filter wheel or a filter slider. By changing excitation filters one seeks to excite each fluorochrome in succession, capturing a single image and combining those images with the help of imaging software. There can be some bleeding of one fluorochrome into the image of a shorter wavelength fluorochrome. This is sometimes inevitable, as is the case with Propidium Iodide and FITC.
