Total control
TIRF OVERVIEW
Total Internal Reflection creates an evanescent wave that illuminates only a thin section of your sample: only around 200nm. See your sample with a contrast you never imagined. Â
DRAMATIC INCREASE IN SIGNAL-TO-NOISE RATIO
Elimination of background fluorescence from outside the focal plane can dramatically improve the signal-to-noise ratio and, consequently, the spatial resolution of the features or events of interest.
LOW PHOTOTOXICITY
Only a thin layer of the sample, up to 200nm from the coverslip, is excited so virtually all of the emitting fluorophores are in focus and the background signal is significantly reduced. Illuminated with low penetration reduces the photobleaching effect.
TIRF Products
Review our product line for TIRF applications.
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SINGLE MOLECULE
IMAGING
TIRFm has become a method of choice for single molecule detection. The Cairn OptoTIRF is a great budget-friendly way to add expanded capability to your existing system.
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SUPER-RESOLUTION
(PALM/STORM)
Use TIRF microscopy for PALM/STORM super-resolution imaging. The OptoTIRF is a great budget-friendly way to add expanded capability to your existing system.
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KINETIC
STUDIES
TIRF microscopy is an excellent technique for combining kinetic studies with spatial information. Try out the OptoTIRF to look at your molecule trafficking - you won't believe your eyes.
