Multiphoton image of the periosteum surrounding a young mouse femur. The collagen scaffolding is visualized by second harmonic generation (green) and the osteocytic fibroblasts, loaded with the cellular indicator dye calcein-AM (red), are visualized with two-photon excited fluorescence. The excitation wavelength is 920nm.
Rebecca Williams, Warren Zipfel, Watt W. Webb, Cornell University.
Chroma Technology Corp