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Mouse Femur

IMAGE DESCRIPTION
Multiphoton image of the periosteum surrounding a young mouse femur. The collagen scaffolding is visualized by second harmonic generation (green) and the osteocytic fibroblasts, loaded with the cellular indicator dye calcein-AM (red), are visualized with two-photon excited fluorescence. The excitation wavelength is 920nm.

IMAGE BY:
Rebecca Williams, Warren Zipfel, Watt W. Webb, Cornell University.

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89 North | Light sources for fluorescence imaging

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