Rat Preovulatory Follicle

Laser scanning confocal photomicrograph of a rat preovulatory follicle showing an oocyte surrounded by granulosa cells within the antral cavity. An individual section of an ovarian follicle is stained with Yo-Pro-1 (Molecular Probes). Whole ovary tissue was fixed in paraformaldehyde, dehydrated in 100% MEOH and cleared in BABB. The focal plane in these pictures was optically sliced at the point of the oocyte’s nucleolus. Magnification is 100x. Images were taken with a 10x (NA 0.3) objective using a Chroma Triple Dichroic filter and a Barrier filter 532/22 on a Leica confocal microscope.

Image by:
Robert M. Zucker, Ph.D.; Jerome M. Goldman, Ph.D. and Sally Perreault Darney, Ph.D., U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Toxicology Assessment Division, Research Triangle Park, NC.

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