Rat thoracic aorta fibroblast cell
The rat thoracic aorta fibroblast cell (A7r5 line) presented in the digital image above was immunofluorescently labeled with mouse anti-alpha-tubulin primary antibodies followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568. In addition, the culture was stained with Alexa Fluor 350 conjugated to phalloidin and SYTOX Green, targeting the filamentous actin network and nuclei, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks (Chroma; DAPI, FITC HQ, and Texas Red HQ). During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.
Michael W. Davidson, Ph.D., National High Magnetic Field Laboratory, Florida State University.
Chroma Technology Corp.