The time portrait
Autofluorescence lifetime imaging is becoming more and more popular for detecting the signal from NAD(P)H, a co-enzyme, which is one of the key participants
in cellular energy metabolism. The technique reveals different states of the compound and thus provides information about the metabolic condition of the cells. This galaxy-like group
of cells is comprised of cervical carcinoma HeLa cells, which were imaged with time-correlated single photon counting, after which the distribution of the lifetimes was color-coded.
Vladimir Ghukasyan, Ph.D., Jim Hsu and Fu-Jen Kao, Ph.D., National Yang-Ming University, Institute of Biophotonics, Taipei, Taiwan.
Chroma Technology Corp.