Yes, we might already make a set that will work well, or we can make a custom set if needed. Filter sets are usually named for the fluorophore for which they are recommended, but can be useful with many others in the same spectral range. Please contact us at firstname.lastname@example.org to discuss the possibilities. Please be ready to provide as much of the following information as possible: excitation (absorption) and emission peak wavelengths, light source, microscope and detector. Also please tell us which other fluorophores will be present in the same sample so that we can help you detect only the desired fluorophore(s).We can then recommend a catalog set, or we can specify a custom set for you.
Most fluorophores have a fairly broad range of wavelengths that will excite them, and a fairly broad emission spectrum. Excitation filters used in fluorescence microscopy need to have a bandwidth of at least 20nm and usually more. In order to accommodate each filter’s transition slope from transmission to blocking, there is typically a 10-12 nm gap between the excitation and emission filter. The emission filter must also transmit a fairly broad range. This gap ensures that the excitation light will not be transmitted by the emission filter. This often requires a shift of the center wavelength (CWL) of the excitation filter to a slightly shorter wavelength than the excitation peak, and a shift of the CWL of the emission filter to a slightly longer wavelength than the emission peak.
However, the wavelength range of filters in recommended filter sets will generally coincide with the peak excitation and emission wavelengths. In cases where this is not possible, the emission spectrum is typically favored over the excitation spectrum, as this minimizes the rate of photobleaching during image acquisition.
Yes, we can. Please contact us with as much information as possible about your application. Helpful information includes things like light source, microscope, detector, all fluorophores present in the same sample, as well as any other details about the application that you know. If the fluorophore is not well known please provide us with the excitation (absorption) and emission wavelengths.
If you have this concern, please contact us to discuss them. The use of multiple fluorophores in a sample, and the choice of filter sets can be challenging. Depending on the desired combination of fluorophores, filters may or may not prevent bleed through. There are also factors other than the optics that should be considered. Please email our applications scientists at email@example.com with details about your application.
Yes. We offer filters to fit all commercial microscopes and home-built systems. Please provide the model of instrument you will be using to ensure that you receive filters of the correct size. If you are using a home-built system please specify the dimensions of each component of the set including any thickness constraints you may have.
There is no one standard size, as many microscopes, flow systems, and plate readers use filters of different sizes. Our most common filter size is 25mm diameter (most holders that call for 1” optics will take our standard 25mm part). Most filters come in a ring that is 3.5mm thick. The most common dichroic sizes are 25.5x36x1mm or 26x38x1mm. We can manufacture optics to almost any physical specification that is required. Please contact us for more information if you don’t see the size you need in our size list when adding a filter to the shopping cart.
Our AT filter sets are a lower cost option. AT filter sets are the perfect choice for routine fluorescence detection and assays, and for most applications requiring accurate color discrimination and excellent out-of-band blocking. Our ET filter sets are our highest quality option; they have zero-pixel-shift image registration, and are necessary for applications requiring the highest possible signal intensities.
In most cases this is not recommended. Our excitation filters are made to block all light from 300-1000nm or higher outside of the bandpass. Most of our emission filters do not block light that far out of the transmission band and therefore might pass some unwanted light from your light source to your sample. More importantly, this unblocked excitation light will then be transmitted to the detector.
Yes, an excitation filter is required. The output of LED light sources is not monochromatic and instead has a Gaussian shaped spectral emission profile. The “tails” of this LED emission often extend >40nm from the peak wavelength and must be excluded from the passband of the emission filter. An excitation filter will block these unwanted wavelengths and transmit only the desired wavelengths to your sample.
Yes, we do recommend an excitation (laser clean up) filter. Few lasers are truly monochromatic. Gas lasers typically emit several discrete wavelengths and the blocking provided by AOTFs is often insufficient to prevent laser noise in the emission channel. Many laser diodes can have significant bandwidth and should be treated as a broadband source. Also, because laser illumination is very intense, relatively low levels of laser light can dramatically affect the signal/noise ratio of a fluorescence image.
No. All of our filter and dichroic designs are very angle sensitive. Our filters are designed for a zero degree angle of incidence (AOI) and dichroics are designed for a 45 degree AOI (unless otherwise specified). Changing this angle will drastically shift the spectral performance of the part.
Yes, the correct dichroic (or polychroic) mirror selection is very important. The job of the dichroic mirror is to reflect the excitation light to the sample and transmit the emission light to the detector.
Yes! Because of the multiplexing typically employed in flow cytometry, you’ll want to use filters which are fully blocked out-of-band up to about 900nm, which is the detection limit of most PMTs. Typically, almost all of our excitation filters and many of our emission filters will work well in these applications. If you do not see what you need please contact us at firstname.lastname@example.org. We make many other parts that are not yet published on the website.
We simply need to know the wavelength range that you want the filters to transmit, the wavelength range of the excitation light (typically laser or LED), and the size of the desired filter.
If your system requires dichroic mirrors for an angle other than 45 degrees please contact us, we can provide those as well.
It is very important that we make sure the emission filter is adequately blocking all of the excitation light transmitted to the sample. When filters are requested that are very close in wavelength we look not only at transmission, but at the optical density (blocking) measurements to be sure there will be no unwanted excitation light being transmitted to your detector.
If the emission filter does not sufficiently block all wavelengths of the excitation light, then this light will be transmitted to the detector. Keep in mind that the intensity of the excitation light is many orders-of-magnitude greater than that of the fluorescence emission from a sample labeled with fluorophores. This can appear as anything from a light colored haze to an almost blindingly bright sea of light, obscuring any fluorescence. Furthermore, unblocked excitation light may lead to destruction of equipment or damage to the user’s eyesight.
Please contact us at email@example.com with all information you can gather about the part. A ringed filter will have the part number and lot number on the side of the ring. Dichroics that were sent unmounted will not be marked, but the package they were sent in will have the part and lot number. Cubes that were assembled at Chroma will have lot numbers printed on the cube, beginning with the letter “C” followed by 5 or 6 digits. If you do not have this information we can show you an example of spectral properties from that same design if the part number is known.
Most filters that have been discontinued from the Chroma Technology catalog can still be found on the website as “Discontinued” products. Filters which were made as custom products are not found on the website.
Excitation (x) filters should be positioned with the arrow pointing toward the specimen, toward the inside of the cube, and away from the light source. Emission (m) filters should be placed with the arrow pointing toward the specimen, toward the inside of the cube, and away from the detector/eye. See the Cleaning Handling and Orientation page.
If you are using a purchase order from your company or institution please email this to firstname.lastname@example.org or fax to 802-428-2525. If you are placing a credit card order it is best to go to our website here: https://www.chroma.com/cart . You can order from a quote online from the chroma.com/cart page and then clicking the blue button to add items from a quote. You will need to enter the quote number and email address the quote was sent to.
These are our catalog filter sets mounted in microscope cubes that have been returned to Chroma for various reasons. These filter sets in cubes have been used minimally or not at all. They are not defective and are inspected and cleaned prior to being listed on our website. They are substantially discounted and are in excellent condition.
The first confirmation is an automated acknowledgement for the information submitted on the website. The second is your order confirmation.This is generated after a customer service representative processes your order.This second confirmation contains your customer service representative's name and contact number, your shipping preference, estimated ship date, and your Chroma Sales Order Number. Please reference this number when making any order inquiries.
Your order confirmation will tell you the day your order is scheduled to ship from Chroma.It will also tell you the shipping method we intend to use.Our standard shipping method in the U.S. is 2 business day service, but overnight service is also available upon request. Orders that are leaving the U.S. are shipped with international priority service, which normally takes 2-3 business days.
Please contact the customer service representative, as listed on your order confirmation from or email email@example.com. Please provide the Chroma SO number or your purchase order number when submitting an inquiry.
Please contact the customer service representative, as listed on your order confirmation from or email firstname.lastname@example.org. Remember to include the Chroma SO number or your purchase order number along with a description of the changes you need to make. Please also keep in mind that if you placed a credit card order through our website we are limited to the changes that can be made, so we may ask you to resubmit your credit card information depending on the situation.
Please contact us at email@example.com and tell us which part you need to return. Our normal process is to credit you for returned items, and to have you place a new order for the replacement parts. We need to know the original purchase order number or Chroma sales order number (both are found on the sales order confirmation email/fax) and which part you wish to return and why, so we may provide you with a RMA number to send it back. Upon receiving this number, we ask that you fill out our RMA form and follow the instructions provided. You can start the return process by submitting the RMA request form here: https://www.chroma.com/support/forms/returns-rma-form
Option 1: Search www.chroma.com for the part number found on the filter holder. We have hundreds of available spectra to view online. Please note that the spectra you view online may not directly reflect the exact optic you are holding, though it will be very similar.
Option 2: Contact firstname.lastname@example.org and provide the number found directly to the right of the upward facing arrow on the filter ring, called our lot number. We can use that number to provide the measured spectra for your specific part.
If you are holding a Chroma-mounted filter cube we can ususally provide spectra for all the optics contained within, provided it has not been modified. for a cube, set lot numbers are denoted as C-##### and can be found on the side of the filter cube.
We want every customer to be happy with their purchase. Therefore, we have a 1 year full satisfaction guarantee on all catalog filters. If you purchase items that do not work for your application please contact us at email@example.com . We help you arrange to return your parts and get you what you need.
We offer a lifetime warranty against manufacturer defects for all catalog sputtered filters, a 5 year warranty for catalog metal sputtered filters and a 3 year warranty on other catalog filters. For full details and more information on all parts please visit our Warranty Page, or download the PDF.