Skip to main content
MENU

Given the severity of the COVID-19 outbreak, Chroma's top priority remains the health and well-being of our employees, customers and greater communities. Learn More.

Home An Employee-Owned Company Producing the World's Finest Optical Filters
  • Browse by Fluorochrome
  • Spectra Viewer
  • Products
    • Overview
    • Optical Filters
    • Complete Filter Sets
    • Filter Accessories
  • OEM/Engineering
  • Company
    • Careers
    • Commitment to Quality
  • News & Events
  • Support
    • Overview
    • Customer Support
    • Technical Support
  • Knowledge & Resources
    • Overview
    • About Fluorescence
    • Image Gallery
  • Contact
Order From Quote
Cart:0
  • Overview
  • About Fluorescence
  • Image Gallery
Knowledge & Resources

Working in Wavelengths

  • Overview
  • About Fluorescence
  • Image Gallery

Electromagnetic Spectrum

Figure 1: Visualization of the electromagnetic spectrum, quantified using wavelengths, with the visible region highlighted

Any portion of the electromagnetic spectrum is qualitatively defined by the wavelength of light that comprises it. In other words, the wavelength of a light wave tells us almost everything we would want to know about it. In fluorescence microscopy, the nanometer (nm) is the most commonly used unit and the portion of the spectrum that is typically reported as perceptible by humans ranges from about 380nm to 710nm. Although individual subjectivity makes it difficult to quantify, only about 15% of humans can see anything above 680nm, and it is not recommended to view light below 420nm due to the risk of eye damage. The visible spectrum can be broken up into particular ranges of wavelengths; each corresponding to what we call colors: 

  • violet and indigo: 380 - 450 nm
  • blue and aqua: 450 - 500 nm
  • green: 500 - 570 nm
  • yellow and orange: 570 - 610 nm
  • red: 610 - 710 nm

The wavelengths that direcly flank the visible spectrum are also useful to the world of fluorescence. They are comprised of the short-wavelength band from 320 to 400 nm (near-UV) and the long-wavelength band from 750 to approximately 2500 nm (near-IR). 

In optics, wavelength is often chosen over frequency to quantify light, but they are both proportional and either is accurate. Wavelength (and therefore frequency) is also directly proportional to a wave’s energy, as originally described by Max Planck with the following equation:

  • E = hc/λ

Where E is energy, h is Planck’s constant, c is the speed of light, and λ is the wavelength of light. This relationship expresses the idea that light of a shorter wavelength (i.e. violet) has more energy than light of a longer wavelength (i.e. red). 

 

<< Introduction to Fluorescence || The Fluorescence Phenomenon >>

 

 

  • Build a Filter Quote
  • Order From Quote
  • Contact
  • Shipping & Returns

 

 

Chroma Technology Corp | An Employee-Owned Company
89 North | Light sources for fluorescence imaging

© 2020 Chroma Technology Corporation. All rights reserved.
10 Imtec Lane, Bellows Falls, VT 05101 USA
Tel 800.824.7662 • Fax 802.428.2525 •
sales@chroma.com
Media Queries: media@chroma.com
Terms of Use  /  Privacy Policy

ISO 9001:2015 CertifiedANAB Accredited

By using this website, you agree to our use of cookies. We use cookies to improve the user experience and to help our website run effectively. To learn more, read our Privacy Policy.