Ratiometric imaging uses a ratio of images to determine ion concentration, voltage or pH levels in a cell of a cell network. One common example is the Fura-2 fluorophore, which absorbs light preferentially at 380nm in high intracellular concentrations of calcium and absorbs light preferentially at 340nm in low concentrations of calcium. By exciting the sample at these two wavelengths and taking a ratio of the image, a semi-quantitative determination of calcium levels is possible. Other ratiometric fluorophores, like INDO-1, undergo a change to their emission wavelength under differing ion concentrations.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
Ratiometric Calcium Indicators - Fluorescence Excitation Spectra of Fura2
No Results Found.
Sorry we did not find an results for the criteria you specified.
Please try changing the narrow by filters on the left, or the search within keyword field.