Ratiometric imaging uses a ratio of images to determine ion concentration, voltage or pH levels in a cell of a cell network. One common example is the Fura-2 fluorophore, which absorbs light preferentially at 380nm in high intracellular concentrations of calcium and absorbs light preferentially at 340nm in low concentrations of calcium. By exciting the sample at these two wavelengths and taking a ratio of the image, a semi-quantitative determination of calcium levels is possible. Other ratiometric fluorophores, like INDO-1, undergo a change to their emission wavelength under differing ion concentrations.
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Ratiometric Calcium Indicators - Fluorescence Excitation Spectra of Fura2
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes