Ratiometric
Ratiometric imaging uses a ratio of images to determine ion concentration, voltage or pH levels in a cell of a cell network. One common example is the Fura-2 fluorophore, which absorbs light preferentially at 380nm in high intracellular concentrations of calcium and absorbs light preferentially at 340nm in low concentrations of calcium. By exciting the sample at these two wavelengths and taking a ratio of the image, a semi-quantitative determination of calcium levels is possible. Other ratiometric fluorophores, like INDO-1, undergo a change to their emission wavelength under differing ion concentrations.
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Ratiometric Calcium Indicators - Fluorescence Excitation Spectra of Fura2
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Product Type
Application
Center/Edge/Notch Wavelength
79001
NC1060763 - ET - Fura 2
- While supplies last
- No modifications on size or coating
- Covered by normal warranty
- Normal support provided
79001
NC1135824 - ET - Fura 2
Premounted in a Leica DMi8 standard filter cube, for 25mm filters, fits 1mm-thick dichroics (91050)
Qty in Stock: 1
- While supplies last
- No modifications on size or coating
- Covered by normal warranty
- Normal support provided
