Ratiometric
Ratiometric imaging uses a ratio of images to determine ion concentration, voltage or pH levels in a cell of a cell network. One common example is the Fura-2 fluorophore, which absorbs light preferentially at 380nm in high intracellular concentrations of calcium and absorbs light preferentially at 340nm in low concentrations of calcium. By exciting the sample at these two wavelengths and taking a ratio of the image, a semi-quantitative determination of calcium levels is possible. Other ratiometric fluorophores, like INDO-1, undergo a change to their emission wavelength under differing ion concentrations.
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Ratiometric Calcium Indicators - Fluorescence Excitation Spectra of Fura2
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Application
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- (-) Remove Ratiometric filter Ratiometric
Center/Edge/Notch Wavelength
79001
NC1180920 - ET - Fura 2
Premounted in a BX2 filter cube (U-MF2) For Olympus BX2 and IX2 models (91018)
Qty in Stock: 1
Filters in this Set | Price | ||
---|---|---|---|
ET340x (EX) | $ | 365.00 | |
ET380x (EX) | $ | 365.00 | |
T400lp (BS) | $ | 250.00 | |
ET510/80m (EM) | $ | 365.00 | |
New Set Price | $ | 1,150.00 | |
New Cube Price | $ | 578.00 | |
New Set/Cube Cost | $ | 579.00 | |
Reclaimed Cube Cost | $ | 833.30 |
- While supplies last
- No modifications on size or coating
- Covered by normal warranty
- Normal support provided