TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
Showing 2 products
- (-) Remove TIRF filter TIRF
- (-) Remove Rhodamine 6G filter Rhodamine 6G
- (-) Remove Reclaimed Cubes filter Reclaimed Cubes
Product Type
Fluorochrome
- Alexa Fluor 532™ (2) Apply Alexa Fluor 532™ filter
- Alexa Fluor 647™ (2) Apply Alexa Fluor 647™ filter
- Allophycocyanin (APC) (2) Apply Allophycocyanin (APC) filter
- Atto 647N (2) Apply Atto 647N filter
- CAL Fluor® Gold 540 (2) Apply CAL Fluor® Gold 540 filter
- CAL Fluor® Orange 560 (2) Apply CAL Fluor® Orange 560 filter
- Cy5™ (2) Apply Cy5™ filter
- DiD (2) Apply DiD filter
- Draq5 (2) Apply Draq5 filter
- DyLight 649 (2) Apply DyLight 649 filter
- Eosin (2) Apply Eosin filter
- HEX,SE (2) Apply HEX,SE filter
- MitoTracker Deep Red 633/MeOH (2) Apply MitoTracker Deep Red 633/MeOH filter
- Propidium Iodide (2) Apply Propidium Iodide filter
- Quasar® 670 (2) Apply Quasar® 670 filter
- (-) Remove Rhodamine 6G filter Rhodamine 6G
- SYTO® 60 (2) Apply SYTO® 60 filter
- TO-PRO™-3 (2) Apply TO-PRO™-3 filter
- ZsYellow (2) Apply ZsYellow filter
- mKO (2) Apply mKO filter
- mOrange2 (2) Apply mOrange2 filter
Application
- (-) Remove Fluorescence filter Fluorescence
Center/Edge/Notch Wavelength
TRF59907
NC806191 - ET – 532/640nm Laser Dual Band Set for TIRF applications
Premounted in a Laser TIRF for Nikon TE2000/Ti (91032)
Qty in Stock: 1
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Filters in this Set | Price | ||
---|---|---|---|
ZET532/640x (EX) | $ | 500.00 | |
ZT532/640rpc (BS) | $ | 525.00 | |
ZET532/640m-TRF (EM) | $ | 875.00 | |
New Set Price | $ | 1,800.00 | |
New Cube Price | $ | 550.00 | |
New Set/Cube Cost | $ | 551.00 | |
Reclaimed Cube Cost | $ | 1,235.00 |
- While supplies last
- No modifications on size or coating
- Covered by normal warranty
- Normal support provided
TRF59907
NC892950 - ET – 532/640nm Laser Dual Band Set for TIRF applications
Premounted in a Laser TIRF for Olympus BX2 models (91041)
Qty in Stock: 1
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Filters in this Set | Price | ||
---|---|---|---|
ZET532/640x (EX) | $ | 500.00 | |
ZT532/640rpc (BS) | $ | 525.00 | |
ZET532/640m-TRF (EM) | $ | 875.00 | |
New Set Price | $ | 1,800.00 | |
New Cube Price | $ | 675.00 | |
New Set/Cube Cost | $ | 676.00 | |
Reclaimed Cube Cost | $ | 1,322.50 |
- While supplies last
- No modifications on size or coating
- Covered by normal warranty
- Normal support provided