TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
Showing 2 products
- (-) Remove TIRF filter TIRF
- (-) Remove Alexa Fluor 546™ filter Alexa Fluor 546™
- (-) Remove DiI filter DiI
- (-) Remove Two Bands filter Two Bands
- (-) Remove Catalog Sets filter Catalog Sets
Product Type
Fluorochrome
- Acridine Orange + DNA (2) Apply Acridine Orange + DNA filter
- Alexa Fluor 488™ (2) Apply Alexa Fluor 488™ filter
- (-) Remove Alexa Fluor 546™ filter Alexa Fluor 546™
- Alexa Fluor 555™ (2) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (2) Apply Alexa Fluor 568™ filter
- AsRed2 (2) Apply AsRed2 filter
- Atto 488 (2) Apply Atto 488 filter
- Atto 550 (2) Apply Atto 550 filter
- Azami Green (2) Apply Azami Green filter
- BCECF/pH 9.0 (2) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (2) Apply BODIPY FL/pH7.2 filter
- CAL Fluor® Red 590 (2) Apply CAL Fluor® Red 590 filter
- Calcein (2) Apply Calcein filter
- Cy2™ (2) Apply Cy2™ filter
- Cy3.5™ (2) Apply Cy3.5™ filter
- Cy3™ (2) Apply Cy3™ filter
- (-) Remove DiI filter DiI
- DiO (2) Apply DiO filter
- DsRed (2) Apply DsRed filter
- DyLight 488 (2) Apply DyLight 488 filter
- DyLight 549 (2) Apply DyLight 549 filter
- EGFP (2) Apply EGFP filter
- Emerald GFP (2) Apply Emerald GFP filter
- FAM (2) Apply FAM filter
- FITC (2) Apply FITC filter
- FM™ 1-43 (2) Apply FM™ 1-43 filter
- Fluo-3 (2) Apply Fluo-3 filter
- Fluo-4 (2) Apply Fluo-4 filter
- FusionRed (2) Apply FusionRed filter
- GFP (2) Apply GFP filter
- LysoTracker Green/pH 5.2 (2) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (2) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (2) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Green FM/MeOH (2) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Orange/MeOH (2) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (2) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (2) Apply Oregon Green™ 488 filter
- Propidium Iodide (2) Apply Propidium Iodide filter
- Quasar® 570 (2) Apply Quasar® 570 filter
- R-phycoerythrin (2) Apply R-phycoerythrin filter
- ROX (2) Apply ROX filter
- Rhod-2 (2) Apply Rhod-2 filter
- Rhodamine Red™-X (2) Apply Rhodamine Red™-X filter
- SYBR® Green I (2) Apply SYBR® Green I filter
- SYTO 9/DNA (2) Apply SYTO 9/DNA filter
- TAMRA (2) Apply TAMRA filter
- TRITC (2) Apply TRITC filter
- TagRFP (2) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (2) Apply Tetramethylrhodamine isothiocyanate filter
- X-rhod-1/Ca2+ (2) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (2) Apply ZsGreen1 filter
- mWasabi (2) Apply mWasabi filter
- tdTomato (2) Apply tdTomato filter
Application
Center/Edge/Notch Wavelength
Emission passbands
TRF59904
ET – 488/561nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET488/561x (EX) | $ | 500.00 | |
ZT488/561rpc (BS) | $ | 625.00 | |
ZET488/561m-TRF (EM) | $ | 875.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,350.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,475.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,225.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,475.00 |
TRF59908-EM
ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
Set includes the same filter combination as the 59908 PALM set, but with the 2mm TIRF dichroic.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET405/488/561x (EX) | $ | 550.00 | |
ZT405/488/561rpc (BS) | $ | 650.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET575lp (EM) | $ | 365.00 | |
ZET488/561m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,750.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,875.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,625.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,875.00 |