TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
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- (-) Remove TIRF filter TIRF
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- (-) Remove DsRed filter DsRed
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Product Type
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Fluorochrome
- Alexa Fluor 514™ (2) Apply Alexa Fluor 514™ filter
- Alexa Fluor 546™ (2) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (2) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (2) Apply Alexa Fluor 568™ filter
- AsRed2 (2) Apply AsRed2 filter
- Atto 425 (2) Apply Atto 425 filter
- Atto 550 (2) Apply Atto 550 filter
- Brilliant Violet™ 480 (2) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Red 590 (2) Apply CAL Fluor® Red 590 filter
- CFP (2) Apply CFP filter
- (-) Remove Calcium Green™-1 filter Calcium Green™-1
- Cerulean (2) Apply Cerulean filter
- Citrine (2) Apply Citrine filter
- Cy3.5™ (2) Apply Cy3.5™ filter
- Cy3™ (2) Apply Cy3™ filter
- DiI (2) Apply DiI filter
- (-) Remove DsRed filter DsRed
- DyLight 549 (2) Apply DyLight 549 filter
- ECFP (2) Apply ECFP filter
- EYFP/pH 7 (2) Apply EYFP/pH 7 filter
- FM™ 4-64 (2) Apply FM™ 4-64 filter
- FlAsH-CCPFCC (2) Apply FlAsH-CCPFCC filter
- Fluo-3 (2) Apply Fluo-3 filter
- FusionRed (2) Apply FusionRed filter
- Lucifer Yellow (2) Apply Lucifer Yellow filter
- LysoTracker Red/pH 5.2 (2) Apply LysoTracker Red/pH 5.2 filter
- MitoTracker Orange/MeOH (2) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (2) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 514 (2) Apply Oregon Green™ 514 filter
- Propidium Iodide (2) Apply Propidium Iodide filter
- Quasar® 570 (2) Apply Quasar® 570 filter
- R-phycoerythrin (2) Apply R-phycoerythrin filter
- ROX (2) Apply ROX filter
- Rhod-2 (2) Apply Rhod-2 filter
- Rhodamine 123 (2) Apply Rhodamine 123 filter
- Rhodamine Red™-X (2) Apply Rhodamine Red™-X filter
- TAMRA (2) Apply TAMRA filter
- TRITC (2) Apply TRITC filter
- TagRFP (2) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (2) Apply Tetramethylrhodamine isothiocyanate filter
- Topaz (2) Apply Topaz filter
- X-rhod-1/Ca2+ (2) Apply X-rhod-1/Ca2+ filter
- mCitrine (2) Apply mCitrine filter
- mKeima Red (2) Apply mKeima Red filter
- tdTomato (2) Apply tdTomato filter
Application
Center/Edge/Notch Wavelength
TRF69904
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET442/514/561x (EX) | $ | 550.00 | |
ZT442/514/561rpc (BS) | $ | 650.00 | |
ZET442/514/561m-TRF (EM) | $ | 925.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,450.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,575.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,325.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,575.00 |
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET442/514/561x (EX) | $ | 550.00 | |
ZT442/514/561rpc (BS) | $ | 650.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET540/30m (EM) | $ | 365.00 | |
ET575lp (EM) | $ | 365.00 | |
ZET442/514/561m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |