TIRF & TRF69900 Series
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
We offer two versions of each our TRF69900 triple band TIRF filter sets, each providing greater levels of blocking of the TIRF lasers than our 69900 series filter sets. One version (“-EM”) utilizes additional, single band emission filters which match the Triple band emission filter in the filter cube. This option requires an emission filter wheel to house the additional emission filters. The other option relies on one triple band emission filter which provides extra blocking of the TIRF laser, and is housed in the filter cube. The highest signal/noise levels are obtained with the “-EM” versions, however for workers who need very fast switching or who don’t have a filter wheel, the alternate version is an excellent option. The Ultra-Flat Dichroics in our TIRF sets are 2mm thick for distortion-free reflection of TIRF lasers with surface flatness =/> 0.5 waves/inch P-V, RWD. All catalog TIRF sets are mounted in Chroma’s metal TIRF cubes. For more information, please see the supporting materials on our TIRF filter set webpages.
Showing 4 products
- (-) Remove TIRF filter TIRF
- (-) Remove DiI filter DiI
- (-) Remove TRF69900 Series filter TRF69900 Series
- (-) Remove Catalog Sets filter Catalog Sets
Product Type
- (-) Remove Catalog Sets filter Catalog Sets
Fluorochrome
- Acridine Orange + DNA (2) Apply Acridine Orange + DNA filter
- Alexa Fluor 350™ (2) Apply Alexa Fluor 350™ filter
- Alexa Fluor 405 (2) Apply Alexa Fluor 405 filter
- Alexa Fluor 488™ (2) Apply Alexa Fluor 488™ filter
- Alexa Fluor 514™ (2) Apply Alexa Fluor 514™ filter
- Alexa Fluor 546™ (4) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (4) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (4) Apply Alexa Fluor 568™ filter
- AsRed2 (4) Apply AsRed2 filter
- Atto 425 (2) Apply Atto 425 filter
- Atto 488 (2) Apply Atto 488 filter
- Atto 550 (4) Apply Atto 550 filter
- Azami Green (2) Apply Azami Green filter
- BCECF/pH 9.0 (2) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (2) Apply BODIPY FL/pH7.2 filter
- Biosearch Blue (2) Apply Biosearch Blue filter
- Brilliant Violet™ 421 (2) Apply Brilliant Violet™ 421 filter
- Brilliant Violet™ 480 (2) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Red 590 (4) Apply CAL Fluor® Red 590 filter
- CFP (2) Apply CFP filter
- Calcein (2) Apply Calcein filter
- Calcium Green™-1 (2) Apply Calcium Green™-1 filter
- Cerulean (2) Apply Cerulean filter
- Citrine (2) Apply Citrine filter
- Cy2™ (2) Apply Cy2™ filter
- Cy3.5™ (4) Apply Cy3.5™ filter
- Cy3™ (4) Apply Cy3™ filter
- DAPI (2) Apply DAPI filter
- (-) Remove DiI filter DiI
- DiO (2) Apply DiO filter
- DsRed (4) Apply DsRed filter
- DyLight 350 (2) Apply DyLight 350 filter
- DyLight 405 (2) Apply DyLight 405 filter
- DyLight 488 (2) Apply DyLight 488 filter
- DyLight 549 (4) Apply DyLight 549 filter
- EBFP2 (2) Apply EBFP2 filter
- ECFP (2) Apply ECFP filter
- EGFP (2) Apply EGFP filter
- EYFP/pH 7 (2) Apply EYFP/pH 7 filter
- Emerald GFP (2) Apply Emerald GFP filter
- FAM (2) Apply FAM filter
- FITC (2) Apply FITC filter
- FM™ 1-43 (2) Apply FM™ 1-43 filter
- FM™ 4-64 (2) Apply FM™ 4-64 filter
- FlAsH-CCPFCC (2) Apply FlAsH-CCPFCC filter
- Fluo-3 (4) Apply Fluo-3 filter
- Fluo-4 (2) Apply Fluo-4 filter
- FusionRed (4) Apply FusionRed filter
- GFP (2) Apply GFP filter
- Hoechst 33258 (2) Apply Hoechst 33258 filter
- Lucifer Yellow (2) Apply Lucifer Yellow filter
- LysoTracker Blue/MeOH (2) Apply LysoTracker Blue/MeOH filter
- LysoTracker Green/pH 5.2 (2) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (4) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (2) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Green FM/MeOH (2) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Orange/MeOH (4) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (4) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (2) Apply Oregon Green™ 488 filter
- Oregon Green™ 514 (2) Apply Oregon Green™ 514 filter
- Pacific Blue (2) Apply Pacific Blue filter
- Propidium Iodide (4) Apply Propidium Iodide filter
- Qdot 525 (2) Apply Qdot 525 filter
- Qdot 585 (2) Apply Qdot 585 filter
- Qdot 605 (2) Apply Qdot 605 filter
- Quasar® 570 (4) Apply Quasar® 570 filter
- R-phycoerythrin (4) Apply R-phycoerythrin filter
- ROX (4) Apply ROX filter
- Rhod-2 (4) Apply Rhod-2 filter
- Rhodamine 123 (2) Apply Rhodamine 123 filter
- Rhodamine Red™-X (4) Apply Rhodamine Red™-X filter
- SYBR® Green I (2) Apply SYBR® Green I filter
- SYTO 9/DNA (2) Apply SYTO 9/DNA filter
- T-Sapphire (2) Apply T-Sapphire filter
- TAMRA (4) Apply TAMRA filter
- TRITC (4) Apply TRITC filter
- TagBFP (2) Apply TagBFP filter
- TagRFP (4) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (4) Apply Tetramethylrhodamine isothiocyanate filter
- Topaz (2) Apply Topaz filter
- X-rhod-1/Ca2+ (4) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (2) Apply ZsGreen1 filter
- mCitrine (2) Apply mCitrine filter
- mKeima Red (2) Apply mKeima Red filter
- mWasabi (2) Apply mWasabi filter
- tdTomato (4) Apply tdTomato filter
Application
Chroma Series Number
- (-) Remove TRF69900 Series filter TRF69900 Series
Center/Edge/Notch Wavelength
TRF69901
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET405/488/561x (EX) | $ | 550.00 | |
ZT405/488/561rpc (BS) | $ | 650.00 | |
ZET405/488/561m-TRF (EM) | $ | 925.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,450.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,575.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,325.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,575.00 |
TRF69901-EM
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET405/488/561x (EX) | $ | 550.00 | |
ZT405/488/561rpc (BS) | $ | 650.00 | |
ET450/50m (EM) | $ | 365.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET575lp (EM) | $ | 365.00 | |
ZET405/488/561m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |
TRF69904
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET442/514/561x (EX) | $ | 550.00 | |
ZT442/514/561rpc (BS) | $ | 650.00 | |
ZET442/514/561m-TRF (EM) | $ | 925.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,450.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,575.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,325.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,575.00 |
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET442/514/561x (EX) | $ | 550.00 | |
ZT442/514/561rpc (BS) | $ | 650.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET540/30m (EM) | $ | 365.00 | |
ET575lp (EM) | $ | 365.00 | |
ZET442/514/561m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |