TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
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Product Type
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Fluorochrome
- Alexa Fluor 532™ (2) Apply Alexa Fluor 532™ filter
- Atto 425 (2) Apply Atto 425 filter
- Brilliant Violet™ 480 (2) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Gold 540 (2) Apply CAL Fluor® Gold 540 filter
- CAL Fluor® Orange 560 (2) Apply CAL Fluor® Orange 560 filter
- CFP (2) Apply CFP filter
- Cerulean (2) Apply Cerulean filter
- ECFP (2) Apply ECFP filter
- (-) Remove Eosin filter Eosin
- HEX,SE (2) Apply HEX,SE filter
- (-) Remove Lucifer Yellow filter Lucifer Yellow
- Propidium Iodide (2) Apply Propidium Iodide filter
- Rhodamine 6G (2) Apply Rhodamine 6G filter
- ZsYellow (2) Apply ZsYellow filter
- mKO (2) Apply mKO filter
- mKeima Red (2) Apply mKeima Red filter
- mOrange2 (2) Apply mOrange2 filter
Application
Center/Edge/Notch Wavelength
TRF59903
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET442/532x (EX) | $ | 500.00 | |
ZT442/532rpc (BS) | $ | 625.00 | |
ZET442/532m-TRF (EM) | $ | 875.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,350.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,475.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,225.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,475.00 |
TRF59903-EM
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET442/532x (EX) | $ | 500.00 | |
ZT442/532rpc (BS) | $ | 625.00 | |
ET500/40m (EM) | $ | 365.00 | |
ET585/65m (EM) | $ | 365.00 | |
ZET442/532m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,650.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,775.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,525.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,775.00 |