TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
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- (-) Remove TIRF filter TIRF
- (-) Remove FM™ 4-64 filter FM™ 4-64
- (-) Remove Texas Red® filter Texas Red®
- (-) Remove Catalog Sets filter Catalog Sets
Product Type
- (-) Remove Catalog Sets filter Catalog Sets
Fluorochrome
- Alexa Fluor 514™ (2) Apply Alexa Fluor 514™ filter
- Alexa Fluor 568™ (2) Apply Alexa Fluor 568™ filter
- Alexa Fluor 594™ (2) Apply Alexa Fluor 594™ filter
- AsRed2 (2) Apply AsRed2 filter
- Atto 425 (2) Apply Atto 425 filter
- Brilliant Violet™ 480 (2) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Red 590 (2) Apply CAL Fluor® Red 590 filter
- CAL Fluor® Red 610 (2) Apply CAL Fluor® Red 610 filter
- CFP (2) Apply CFP filter
- Calcium Green™-1 (2) Apply Calcium Green™-1 filter
- Cerulean (2) Apply Cerulean filter
- Citrine (2) Apply Citrine filter
- Cy3.5™ (2) Apply Cy3.5™ filter
- DyLight 594 (2) Apply DyLight 594 filter
- ECFP (2) Apply ECFP filter
- EYFP/pH 7 (2) Apply EYFP/pH 7 filter
- (-) Remove FM™ 4-64 filter FM™ 4-64
- FlAsH-CCPFCC (2) Apply FlAsH-CCPFCC filter
- Fluo-3 (2) Apply Fluo-3 filter
- FusionRed (2) Apply FusionRed filter
- Killer Red (2) Apply Killer Red filter
- Lucifer Yellow (2) Apply Lucifer Yellow filter
- LysoTracker Red/pH 5.2 (2) Apply LysoTracker Red/pH 5.2 filter
- MitoTracker Red/MeOH (2) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 514 (2) Apply Oregon Green™ 514 filter
- ROX (2) Apply ROX filter
- ReAsH-CCPGCC (2) Apply ReAsH-CCPGCC filter
- Resorufin (2) Apply Resorufin filter
- Rhodamine 123 (2) Apply Rhodamine 123 filter
- Rhodamine Red™-X (2) Apply Rhodamine Red™-X filter
- SNARF pH 9.0 (2) Apply SNARF pH 9.0 filter
- Sulforhodamine 101 (2) Apply Sulforhodamine 101 filter
- (-) Remove Texas Red® filter Texas Red®
- Texas Red®-X (2) Apply Texas Red®-X filter
- Topaz (2) Apply Topaz filter
- TurboFP650 (2) Apply TurboFP650 filter
- X-rhod-1/Ca2+ (2) Apply X-rhod-1/Ca2+ filter
- mCherry (2) Apply mCherry filter
- mCitrine (2) Apply mCitrine filter
- mKate2 (2) Apply mKate2 filter
- mKeima Red (2) Apply mKeima Red filter
- mPlum (2) Apply mPlum filter
- mRFP1 (2) Apply mRFP1 filter
Application
Center/Edge/Notch Wavelength
TRF69905
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET445/514/594x (EX) | $ | 550.00 | |
ZT445/514/594rpc (BS) | $ | 650.00 | |
ZET445/514/594m-TRF (EM) | $ | 925.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,450.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,575.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,325.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,575.00 |
TRF69905-EM
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
Dichroic beamsplitters for all TIRF filter cubes are 2mm thick
Filters in this Set | Price | ||
---|---|---|---|
ZET445/514/594x (EX) | $ | 550.00 | |
ZT445/514/594rpc (BS) | $ | 650.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET550/50m (EM) | $ | 365.00 | |
ET645/75m (EM) | $ | 365.00 | |
ZET445/514/594m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |