TRF49909 - ET - 561nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.

All transmission and blocking (OD) data are actual, measured spectra of representative production lots. Spectra varies slightly from lot to lot. Optical density values in excess of 6 may appear noisy because such evaluations push the resolution limit of low light level measurements.
- Legend
- Fluorochromes
title | em | ex | |
---|---|---|---|
Abberior Cage 500 | 525nm | 504nm | |
Abberior Cage 532 | 540nm | 519nm | |
Abberior Cage 590 | 607nm | 586nm | |
Abberior Cage 635 | 647nm | 630nm | |
Abberior Flip 565 | 584nm | 558nm | |
Abberior Live 510 | 527nm | 502nm | |
Abberior Live 515 | 544nm | 517nm | |
Abberior Live 580 | 632nm | 603nm | |
Abberior rsEGFP | 510nm | 492nm | |
Abberior rsEGFP2 | 502nm | 483nm | |
Abberior Star 440SXP | 512nm | 448nm | |
Abberior Star 470SXP | 625nm | 470nm | |
Abberior Star 488 | 525nm | 500nm | |
Abberior Star 512 | 534nm | 512nm | |
Abberior Star 520SXP | 633nm | 520nm | |
Abberior Star 600 | 626nm | 604nm | |
Abberior Star 635 | 659nm | 640nm | |
Abberior Star 635p | 654nm | 633nm | |
Abberior Star Red | 659nm | 637nm | |
Acridine Orange + DNA | 526nm | 500nm | |
Alexa Fluor 350™ | 442nm | 346nm | |
Alexa Fluor 405 | 422nm | 401nm | |
Alexa Fluor 488™ | 520nm | 498nm | |
Alexa Fluor 514™ | 539nm | 517nm | |
Alexa Fluor 532™ | 554nm | 531nm | |
Alexa Fluor 546™ | 573nm | 556nm | |
Alexa Fluor 555™ | 568nm | 553nm | |
Alexa Fluor 568™ | 603nm | 578nm | |
Alexa Fluor 594™ | 617nm | 590nm | |
Alexa Fluor 633™ | 647nm | 632nm | |
Alexa Fluor 647™ | 666nm | 649nm | |
Alexa Fluor 680™ | 702nm | 680nm | |
Alexa Fluor 700™ | 719nm | 696nm | |
Alexa Fluor 750™ | 779nm | 752nm | |
Alexa Fluor 790™ | 805nm | 782nm | |
Allophycocyanin (APC) | 660nm | 650nm | |
AmCyan | 489nm | 458nm | |
AsRed2 | 592nm | 576nm | |
Atto 390 | 479nm | 390nm | |
Atto 425 | 485nm | 436nm | |
Atto 465 | 507nm | 453nm | |
Atto 488 | 525nm | 501nm | |
Atto 550 | 576nm | 554nm | |
Atto 633 | 657nm | 630nm | |
Atto 647N | 669nm | 644nm | |
Atto 680 | 700nm | 680nm | |
Azami Green | 505nm | 492nm | |
BB515 | 514nm | 490nm | |
BCECF/pH 5.2 | 520nm | 482nm | |
BCECF/pH 9.0 | 528nm | 503nm | |
Biosearch Blue | 447nm | 352nm | |
BODIPY FL/pH7.2 | 512nm | 505nm | |
Brilliant Violet™ 421 | 422nm | 408nm | |
Brilliant Violet™ 480 | 478nm | 437nm | |
Brilliant Violet™ 510 | 512nm | 405nm | |
Brilliant Violet™ 570 | 573nm | 407nm | |
Brilliant Violet™ 605 | 605nm | 407nm | |
Brilliant Violet™ 650 | 649nm | 407nm | |
Brilliant Violet™ 711 | 713nm | 407nm | |
Brilliant Violet™ 750 | 750nm | 407nm | |
Brilliant Violet™ 786 | 786nm | 408nm | |
Brilliant™ Ultraviolet 395 | 395nm | 348nm | |
Brilliant™ Ultraviolet 496 | 498nm | 348nm | |
Brilliant™ Ultraviolet 661 | 661nm | 348nm | |
Brilliant™ Ultraviolet 737 | 735nm | 350nm | |
Brilliant™ Ultraviolet 805 | 805nm | 350nm | |
CAL Fluor® Gold 540 | 544nm | 522nm | |
CAL Fluor® Orange 560 | 559nm | 538nm | |
CAL Fluor® Red 590 | 591nm | 569nm | |
CAL Fluor® Red 610 | 610nm | 590nm | |
CAL Fluor® Red 635 | 637nm | 618nm | |
Calcein | 517nm | 494nm | |
Calcium Green™-1 | 531nm | 506nm | |
Cerulean | 475nm | 433nm | |
CFP | 475nm | 433nm | |
Citrine | 529nm | 514nm | |
Coumarin | 470nm | 384nm | |
Cy2™ | 506nm | 489nm | |
Cy3.5™ | 598nm | 581nm | |
Cy3™ | 570nm | 552nm | |
Cy5.5™ | 695nm | 675nm | |
Cy5™ | 670nm | 649nm | |
Cy7™ | 767nm | 743nm | |
DAPI | 461nm | 359nm | |
Di-8-ANEPPS non-ratiometric | 620nm | 530nm | |
DiA | 591nm | 456nm | |
DiD | 665nm | 644nm | |
DiI | 569nm | 551nm | |
DiO | 502nm | 484nm | |
DiR | 780nm | 748nm | |
Draq5 | 683nm | 647nm | |
DsRed | 584nm | 557nm | |
DyLight 350 | 435nm | 352nm | |
DyLight 405 | 433nm | 399nm | |
DyLight 488 | 517nm | 492nm | |
DyLight 549 | 569nm | 554nm | |
DyLight 594 | 616nm | 592nm | |
DyLight 633 | 645nm | 623nm | |
DyLight 649 | 667nm | 652nm | |
DyLight 680 | 705nm | 677nm | |
DyLight 750 | 772nm | 751nm | |
DyLight 800 | 795nm | 770nm | |
EBFP2 | 448nm | 385nm | |
ECFP | 477nm | 434nm | |
EGFP | 507nm | 488nm | |
Emerald GFP | 510nm | 489nm | |
Eosin | 545nm | 524nm | |
Ethidium Bromide | 603nm | 520nm | |
Ethidium homidimer-1/DNA | 617nm | 527nm | |
EYFP/pH 7 | 527nm | 514nm | |
FAM | 518nm | 492nm | |
FITC | 525nm | 490nm | |
FlAsH-CCPFCC | 530nm | 511nm | |
Fluo-3 | 526nm | 505nm | |
Fluo-4 | 516nm | 494nm | |
FM™ 1-43 | 598nm | 479nm | |
FM™ 4-64 | 740nm | 515nm | |
Fura-2/Ca2+ - free | 510nm | 340nm | |
Fura-2/Ca2+ - saturated | 510nm | 380nm | |
FusionRed | 603nm | 576nm | |
GFP | 507nm | 488nm | |
HEX,SE | 559nm | 533nm | |
Hoechst 33258 | 461nm | 352nm | |
Indo-1/Ca2+ -free | 475nm | 346nm | |
Indo-1/Ca2+ -saturated | 401nm | 330nm | |
JOE | 548nm | 520nm | |
Killer Red | 611nm | 585nm | |
Li-Cor IRDye® 680LT | 693nm | 674nm | |
Li-Cor IRDye® 800CW | 794nm | 778nm | |
Lucifer Yellow | 540nm | 428nm | |
LysoTracker Blue/MeOH | 425nm | 373nm | |
LysoTracker Green/pH 5.2 | 511nm | 504nm | |
LysoTracker Red/pH 5.2 | 590nm | 577nm | |
LysoTracker Yellow HCK-123 | 563nm | 486nm | |
mCherry | 610nm | 587nm | |
mCitrine | 529nm | 514nm | |
MitoTracker Deep Red 633/MeOH | 665nm | 644nm | |
MitoTracker Green FM/MeOH | 516nm | 490nm | |
MitoTracker Orange/MeOH | 576nm | 551nm | |
MitoTracker Red/MeOH | 599nm | 578nm | |
mKate2 | 634nm | 589nm | |
mKeima Red | 620nm | 440nm | |
mKO | 559nm | 548nm | |
mOrange2 | 562nm | 548nm | |
mPlum | 648nm | 589nm | |
mRFP1 | 607nm | 584nm | |
mTFP1 | 492nm | 462nm | |
mWasabi | 509nm | 493nm | |
NBD X/MeOH | 538nm | 467nm | |
Nile Blue | 660nm | 631nm | |
NirFP | 669nm | 604nm | |
Oregon Green™ 488 | 514nm | 490nm | |
Oregon Green™ 514 | 526nm | 506nm | |
Pacific Blue | 455nm | 405nm | |
Propidium Iodide | 617nm | 536nm | |
Pulsar™ 650 | 650nm | 460nm | |
Quasar® 570 | 566nm | 548nm | |
Quasar® 670 | 670nm | 647nm | |
Quasar® 705 | 705nm | 690nm | |
R-phycoerythrin | 578nm | 565nm | |
ReAsH-CCPGCC | 606nm | 592nm | |
Resorufin | 585nm | 571nm | |
Rhod-2 | 581nm | 552nm | |
Rhodamine 123 | 529nm | 507nm | |
Rhodamine 6G | 555nm | 525nm | |
Rhodamine Red™-X | 590nm | 570nm | |
ROX | 604nm | 578nm | |
SBFI/Na+ -free | 505nm | 340nm | |
SBFI/Na+ -saturated | 505nm | 380nm | |
SNARF pH 9.0 | 641nm | 575nm | |
Sulforhodamine 101 | 605nm | 586nm | |
SYBR® Green I | 522nm | 498nm | |
SYTO 9/DNA | 500nm | 483nm | |
SYTO® 60 | 678nm | 652nm | |
T-Sapphire | 510nm | 398nm | |
TagBFP | 457nm | 402nm | |
TagRFP | 584nm | 556nm | |
TAMRA | 580nm | 555nm | |
tdTomato | 581nm | 554nm | |
TET | 536nm | 521nm | |
Tetramethylrhodamine isothiocyanate | 580nm | 555nm | |
Texas Red® | 620nm | 595nm | |
Texas Red®-X | 615nm | 595nm | |
TO-PRO™-3 | 661nm | 642nm | |
Topaz | 529nm | 514nm | |
TRITC | 580nm | 555nm | |
TurboFP650 | 650nm | 592nm | |
X-rhod-1/Ca2+ | 601nm | 580nm | |
ZsGreen1 | 505nm | 493nm | |
ZsYellow | 539nm | 529nm |
Additional emission filters for exceptional blocking and Ultra-High S/N ratios
No-torque metal cubes and stress-free mounting of dichroics
Thicker, Ultra-Flat dichroics for distortion-free reflection of lasers
Additional emission filters for exceptional blocking and Ultra-High S/N ratios
Because TIRF imaging results in the “Total Internal Reflection” of the excitation laser beam, emission filters are challenged with attenuating an enormous amount of laser illumination returning through a microscope objective lens. In some applications such as single-molecule TIRF, including PALM and STORM imaging in TIRF mode, the ratio of illumination/fluorescence signal could be as high as 1015:1. 1
A dichroic at 45 degree AOI in a standard microscope filter cube will typically provide blocking of approx. OD2 at the laser line. One good laser emission filter in the cube or a filter wheel will offer OD6-OD7. Additional blocking is most often required for optimal image quality in TIRF applications and easily verified by measuring the increased signal/noise ratio obtained when pairing emission filters. This can be accomplished using either a simple bandpass emission filter paired with a longpass emission filter in a cube mounted in the microscope turret, or a multi-band emission filter in a cube paired with single bandpass emission filters in a filter wheel.
Our complete TIRF sets remove the guess work and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. And Chroma’s Ultra-Flat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers to give you the ability to create the perfect evanescent wave.
1. Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642–1645 (2006). Supporting Online Materials
No-torque metal cubes and stress-free mounting of dichroics
Chroma offers our own brand of torque-free, metal microscope cubes to fit current Nikon, Olympus and Zeiss models. These cubes are necessary to house our 2mm or 3mm thick Ultra-Flat laser dichroics, as these thicker dichroics will not fit in the standard microscope manufacturer’s filter cubes.
Our TIRF filter cubes affix the dichroics without the use of springs and clips and are aligned at Chroma using set screws to a precise 45 degree angle of incidence. The alignment may also be adjusted by the customer.
Any mechanical means of holding a dichroic, such as springs or clips, will introduce some degree of pinching or twisting which invariably results in warping of the surface of the dichroic. This will distort the reflected laser beam profile, and will likely create problems for applications requiring critically flat reflective surfaces such as TIRF, STED and other Super-Resolution techniques such as PALM/STORM and structured illumination and some image-splitting applications.
Chroma’s TIRF cubes enable stress-free mounting of dichroics, providing distortion-free reflection of lasers to give you the ability to create the perfect evanescent wave.
Thicker, Ultra-Flat dichroics for distortion-free reflection of lasers
Chroma has manufactured dichroics for applications demanding superior levels of surface flatness for many years. We now offer complete, assembled, catalog filter sets for various applications such as TIRF and other applications where distortion-free reflection is critical. These include dichroic mirrors with surface flatness values of <0.5 waves/inch Peak-Valley RWD (Reflected Wavefront Distortion) and =/<0.1 wave/inch RMS. For explanation, see “How We Specify RWD”, and see our surface flatness specifications below.
In this context, surface flatness relates to curvature, and describes how curved the dichroic surface is. Surface curvature causes convergence or divergence of reflected light waves, depending on whether the surface is concave or convex. This results in reflected wavefront distortion (RWD) of whatever is being reflected: lasers, both in basic imaging applications and in more advanced methods such as TIRF and STED; structured illumination patterns; and reflected images in image-splitting systems.
Sputtered thin-films exert stress on glass and fused silica substrates and warp them into varying degrees of curvature. Chroma has learned how to control this to a large extent by developing a proprietary manufacturing method which minimizes surface curvature. Another factor which reduces surface curvature is the use of thicker substrates which provide greater stiffness and therefore more resistance to the stress exerted by these coatings.
Combining these two elements allows Chroma to specify levels of dichroic surface flatness according to thickness. We offer Chroma’s UltraFlat dichroics ("-UF" suffix) with the following specifications for final, post-coating surface flatness:
Thickness | Surface Flatness | Application |
---|---|---|
1mm thick: | =/< 2 waves/inch Peak-Valley (P-V) | Standard Laser Filter Sets |
2mm thick: | =/< 0.5 waves/inch P-V | TIRF Filter Sets, PALM and STORM |
3mm thick: | =/< 0.25 waves/inch P-V | STED and Structured Illumination |
=/>5mm thick: | Contact us | Custom Applications |
The surface flatness of each lot of our UltraFlat dichroics is measured using laser interferometry. Possibly even more important regarding flatness is how the dichroic is held or housed. Even the flattest optics are warped by varying degrees when held in place by mechanical means. See “Holding Dichroics” below.
Note: All laser dichroic part names begin with "ZT" prefix. Typically, our catalog dichroics for basic epifluorescence widefield applications ("T" prefix) are not controlled for flatness because non-coherent illumination does not require it. However, we do also offer standard widefield dichroics in UltraFlat versions with the specifications listed above. All choices available in shopping cart.
Holding Dichroics
The manner in which dichroics are held or housed in filter cubes can dramatically affect their actual flatness in real world applications.
Major microscope manufacturers generally specify 1mm thick dichroics for their standard filter cubes, and these are often held in place mechanically, by springs or clips. Often, this is sufficient for holding 1mm-thick dichroics flat enough for routine laser applications such as confocal or epi-fluorescence using laser illumination, photo-activation and laser ablation. Our 1mm thick Ultra-Flat laser dichroics at better than 2 waves/inch Peak-Valley RWD provide the required flatness.
However, any mechanical means of holding a dichroic will introduce some degree of pinching or twisting which invariably results in warping of the surface of the dichroic.
For more demanding laser applications such as TIRF or STED, or for structured illumination and some reflected image applications, our thicker, Ultra-Flat dichroics can provide much better results. In order to optimally hold these dichroics, Chroma offers custom-designed and manufactured metal microscope cubes which fit most current microscope models and can accommodate dichroics up to 3mm thick. These cubes affix the dichroics without the use of springs and clips and are aligned at Chroma using set screws to a precise 45 degree angle of incidence. The alignment may also be adjusted by the customer.
For workers with their own holders or mounts, we recommend that you hold by placing minimal pressure on the outside edges, rather than by pinching on the top/bottom surfaces to minimize warping. Call or email us to discuss the range of sizes and thicknesses we can provide.
How We Specify RWD
The RWD parameter we measure is referred to as “Peak-to-Valley” (P-V) deformation, and is expressed in “waves/inch” (or lambda/inch) as determined by laser interferometry. This measures the maximum deformation across the clear aperture of a dichroic, and includes the curvature (Power) plus any surface irregularities.
Industrial standards for surface flatness measurements of flat optics, such as dichroics, conform to ISO standards, and are expressed in terms of interferometric “fringe spacings” or fringes. These are interference patterns which appear as a result of differences in index of refraction between that of the dichroic substrate material and air as a laser is reflected off of the measured surface.
The number of fringes is used to calculate the deviation of the measured surface from that of a reference optical “flat”. We measure this using a wavelength of 633nm, which is the laser most often used in an interferometer.
Occasionally, a filter manufacturer may express surface flatness in terms of radius of curvature (ROC), which in the context of flat optics is a more obscure and confusing metric. ROC is used mainly by lens manufacturers who deal with relatively large values for curvature. As an example of how our flatness specification relates to ROC, consider that a 0.5 wave/inch surface flatness is equivalent to a radius of curvature of 254 meters (or about 830 feet). ROC defines the radius of a sphere with a surface curvature equivalent to that of the measured optic.
Others prefer the parameter of “RMS” (root mean square) which provides a measurement of the uniformity of the surface. Because any distortion to surface flatness as a result of the thin film coatings we use will be spherical distortion, this means that the RMS value will typically be approx. =/< ¼ of the of the P-V value. “RMS” will result in a smaller value than P-V to describe the surface of the same dichroic or mirror.
For the same surface curvature, the various measured values for these parameters vary thus: P-V > Power >> RMS.
Sometimes, P-V flatness is defined over a smaller area, such as a 10mm or 15mm clear aperture. The values listed above use the larger scale of 1 inch which results in a larger value for the same curvature.
The relationship between measurement length and flatness is non-linear. Assuming the deformation is primarily spherical curvature due to coating stress, this can be described by a simple quadratic formula. To calculate the equivalent flatness for a clear aperture of ½ the measured value, the flatness expressed as number of waves will be ¼ of the measured value. As the denominator in the expression (inches) varies by “x”, the numerator (waves) varies by x2. An optic which measures 2 waves/inch P-V will measure 0.5 waves/0.5 inch P-V.
If you prefer the surface flatness expressed as Surface Power or RMS, we will provide this upon request.
Finally, remember that the method of holding or housing the dichroic will greatly influence its actual flatness when used in an imaging system.
- Filter Cube Options
- Overview
- Series Description
- Filter Support
TIRF Filter Cube Options

91032
Laser TIRF for Nikon TE2000/Ti
Exciter (x) = 25mm, Emitter (m) = 25mm, Beamsplitter = 38x26mm. Designed to hold the recommended 2mm dichroics for TIRF applications. Accommodates dichroics up to 3mm for critical applications such as TIRF, structured illumination, super-resolution and image-splitting. Allows for precise alignment of dichroic angle of incidence during assembly by Chroma. **Not a standalone product. Sold with dichroic mirror installed at minimum.

91041
Laser TIRF for Olympus BX2 models
Exciter (x) 25mm, Emitter (m) = 25mm, Beamsplitter = 26x36x2mm. Designed to hold the recommended 2mm dichroics for TIRF applications. Accommodates dichroics up to 3mm for critical applications such as TIRF, structured illumination, super-resolution and image-splitting. Allows for precise alignment of dichroic angle of incidence during assembly by Chroma. **Not a standalone product. Sold with dichroic mirror installed at minimum. Microscope models include IX71 and IX81.

91042
Laser TIRF for Zeiss Axio
Exciter (x) = 25mm, Emitter = 25mm, Beamsplitter = 25.5x36x2mm. Not Adjustable. Designed to accommodate dichroics up to 2mm recommended for critcal applications such as TIRF, structured illumination, super-resolution and image-splitting. **Not a standalone product. Sold with dichroic mirror installed at minimum.

91044
Laser TIRF for Olympus BX3/IX3 models, for 25mm filters
Exciter (x) = 25mm, Emitter (m) = 25mm, Beamsplitter = 26x36mm with a maximum thickness of 3mm. Designed to hold the recommended 2mm dichroics for TIRF applications. Accommodates dichroics up to 3mm for critical applications such as TIRF, structured illumination, super-resolution and image-splitting. Allows for precise alignment of dichroic angle of incidence during assembly by Chroma. **Not a standalone product. Sold with dichroic mirror installed at minimum. Models include IX73 and IX83.
ET - 561nm Laser Bandpass Set for TIRF applications
Instruments: All instrument mounting options are available when adding to cart.
Sizes: Up to 25mm diameter or 26x38mm dichroics. (Contact us for larger sizes)
FILTERS IN THIS SET
Filters in this Set | Price | ||
---|---|---|---|
ZET561/10x (EX) | $ | 325.00 | |
ZT561rdc (BS) | $ | 400.00 | |
ET575lp (EM) | $ | 325.00 | |
ET600/50m (EM) | $ | 325.00 |
COMMENTS:
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.