Olympus BX2 filter cube (U-MF2) for Olympus BX2 and IX2 Assembly Instructions

This guide provides step-by-step instructions for assembling a BX2 filter cube (U-MF2) for the Olympus BX2 and IX2 models (Chroma part number 91018), designed to precisely align optical filters in fluorescence microscopy systems. Proper assembly ensures optimal performance and system reliability.

For those new to handling optical filters, we recommend reviewing our Cleaning & Handling Instructions before assembling your filter cube.

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91018 bx2 filter cube %28u mf2%29 for olympus bx2 and ix2 models kit

91018 BX2/IX2 filter cube

Required filter sizes

Exciter (x)	Ø25mm
Emitter (m)	Ø25mm
Beamsplitter	38 x 26 x 1mm

Required tools

A flat, metal spanner tool is used to tighten the retaining rings. This tool comes with the cube.
You also need a small Phillips-head screwdriver to separate the halves and loosen the clip that holds the beamsplitter down.

Instructions

91018 bx2 filter cube %28u mf2%29 for olympus bx2 and ix2 models 1

Step 1

Separate the cube half by removing the two screws shown in the image.

91018 bx2 filter cube %28u mf2%29 for olympus bx2 and ix2 models 2

Step 2

Loosen the screws on the inside of the cube half with the clip that holds the beam splitter. It is
not necessary to remove the clip; the filter will slide under it.

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Step 3

Mount the beam splitter by sliding it under the clip. The beam splitter should be mounted with the arrow facing up.

Tighten both screws on the clip to secure the beam splitter. Make sure the beam splitter is seated correctly before doing so, or you risk cracking the beam splitter.

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Step 4

Reassemble the cube halves. The exciter filter is inserted into the front port, with the arrow pointing toward the beam splitter.

Screw in the retaining ring with the spanner tool to secure the filter.

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Step 5

Insert the emitter filter into the port at the top of the cube, with the arrow pointing toward the beam splitter.

Screw in the retaining ring with the spanner tool to secure the filter.

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Step 6

Finished Cube

Final Checks

Before installing the assembled cube into your optical system, confirm the following:

Filter Orientation

Verify that all filter arrows are aligned correctly. The beamsplitter arrow should face upward. The exciter and emitter filter arrows should point inward, toward the beam splitter.

Beamsplitter Alignment

Check that the registration tabs and holes between the cube halves are fully engaged. The two halves should meet cleanly, with no visible gaps or misalignment.

Filter Seating

Ensure that the excitation and emission filters are properly installed. Filters should be fully inserted into their respective ports. Retaining rings should be threaded evenly to hold the filters securely, without tilt or cross-threading.

Mechanical Stability

Confirm that the screws joining the cube halves are tightened. The cube should feel stable when handled, with no internal movement or looseness.

Visual Inspection

Inspect all optical surfaces for contamination and use cleanroom practices when handling filters. Refer to Chroma’s Cleaning and Handling Guidelines if cleaning is required.

If you encounter alignment issues, looseness, or other concerns during this inspection, disassemble and repeat the relevant steps. For additional support, please contact Chroma Technical Support.