How to Order Filters
Answers to the following questions are extremely helpful when discussing the appropriate filters or filter sets for your use.
- Which fluorochrome(s) is (are) being used?
- What is the wavelength range of excitation and emission for those fluorochromes with which we are not familiar?
- What is the make and model of the microscope or instrument being used?
- What is the size of the excitation and/or emission filters if a filter wheel is being used in either the excitation or emission path?
- Does the filter need to be image quality or not?
- This is particularly important for absorption-type emission filters.
- What is the maximum thickness possible in the filter mount to be used?
- What type of detector is being used e.g. PMT, CCD, film)?
- What are the specific reflection and transmission requirements for the dichroic beamsplitter?
- Dichroic beamsplitters are designed with various spectral profiles.
- No dichroic beamsplitter reflects all wavelengths short of the cut-on wavelengths, nor can it transmit all wavelengths beyond that point.
- Being as specific as possible will increase your chances of getting the beamsplitter that you require and will diminish the possibility of paying too much for a beamsplitter that is more than you require.
- It is best if we know the excitation and emission filters being used.
- This information is particularly important when ordering a dichroic beamsplitter in the absence of any other filters.
Call 1-800-824-7662 or 802-428-2500
Email orders [at] chroma [dot] com