FRET

Förster  Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon.  The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.

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FRET

 

 

Showing 24 products

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Fluorochrome

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Light Source

Chroma Series Number

Center/Edge/Notch Wavelength

Emission passbands

  • 24 Results Found
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  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59901-EM

ET – 405/488nm Laser Dual Band Set for TIRF applications

For use with lasers between 400-408nm and 485-491nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET405/488x (EX) $ 500.00
ZT405/488rpc (BS) $ 625.00
ET450/50m (EM) $ 365.00
ET525/50m (EM) $ 365.00
ZET405/488m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,650.00
Mounted in a Laser TIRF for Olympus BX2 models $2,775.00
Mounted in a Laser TIRF for Zeiss Axio $2,525.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,775.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF69901-EM

ET - 405/488/561nm Laser Triple Band Set for TIRF applications

For use with lasers between 400-410nm, 486-492nm and 558-563nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET405/488/561x (EX) $ 550.00
ZT405/488/561rpc (BS) $ 650.00
ET450/50m (EM) $ 365.00
ET525/50m (EM) $ 365.00
ET575lp (EM) $ 365.00
ZET405/488/561m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $3,100.00
Mounted in a Laser TIRF for Olympus BX2 models $3,225.00
Mounted in a Laser TIRF for Zeiss Axio $2,975.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $3,225.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF89902-EM

ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET405/488/561/647x (EX) $ 575.00
ZT405/488/561/647rpc (BS) $ 675.00
ET450/50m (EM) $ 365.00
ET525/50m (EM) $ 365.00
ET605/52m (EM) $ 365.00
ET700/50m (EM) $ 365.00
ZET405/488/561/647m (EM) $ 650.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $3,500.00
Mounted in a Laser TIRF for Olympus BX2 models $3,625.00
Mounted in a Laser TIRF for Zeiss Axio $3,375.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $3,625.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF69902-EM

ET - 405/488/594nm Laser Triple Band Set for TIRF applications

For use with lasers between 400-412nm, 486-492nm and 591-596nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET405/488/594x (EX) $ 550.00
ZT405/488/594rpc (BS) $ 650.00
ET450/50m (EM) $ 365.00
ET535/70m (EM) $ 365.00
ET645/75m (EM) $ 365.00
ZET405/488/594m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $3,100.00
Mounted in a Laser TIRF for Olympus BX2 models $3,225.00
Mounted in a Laser TIRF for Zeiss Axio $2,975.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $3,225.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  Filters in this Set   Price
ET402/15x (EX) $ 365.00
ET482/28x (EX) $ 365.00
ET545/25x (EX) $ 365.00
69000bs (BS) $ 425.00
ET455/50m (EM) $ 365.00
ET525/36m (EM) $ 365.00
ET605/70m (EM) $ 365.00
$2,200.00
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
  • Overview
  Filters in this Set   Price
AT350/50x V2 (EX) $ 225.00
ET402/15x (EX) $ 365.00
ET490/20x (EX) $ 365.00
ET555/25x (EX) $ 365.00
ET645/30x (EX) $ 365.00
89100bs (BS) $ 475.00
ET455/50m (EM) $ 365.00
ET525/36m (EM) $ 365.00
ET605/52m (EM) $ 365.00
ET705/72m (EM) $ 365.00
$2,850.00
Discounted Options
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
  • Overview
  Filters in this Set   Price
ET402/15x (EX) $ 365.00
ET490/20x (EX) $ 365.00
ET572/35x (EX) $ 365.00
69002bs (BS) $ 425.00
ET455/50m (EM) $ 365.00
ET535/50m (EM) $ 365.00
ET632/60m (EM) $ 365.00
$2,200.00
Discounted Options
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FRET
  • ET Series
  • White Light

89002

ET - ECFP/EYFP

Best with these fluorochromes

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET430/24x (EX) $ 365.00
ET500/20x (EX) $ 365.00
69008bs (BS) $ 425.00
ET470/24m (EM) $ 365.00
ET535/30m (EM) $ 365.00
$1,600.00
Discounted Options
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FRET
  • ET Series
  • White Light

89007

ET - ECFP/EYFP/DsRed

Best with these fluorochromes

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET430/24x (EX) $ 365.00
ET500/20x (EX) $ 365.00
ET555/20x (EX) $ 365.00
89007bs (BS) $ 425.00
ET470/24m (EM) $ 365.00
ET535/30m (EM) $ 365.00
ET600/50m (EM) $ 365.00
$2,200.00
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FRET
  • ET Series
  • White Light

89006

ET - ECFP/EYFP/mCherry

Best with these fluorochromes

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET430/24x (EX) $ 365.00
ET500/20x (EX) $ 365.00
ET572/35x (EX) $ 365.00
69008bs (BS) $ 425.00
ET470/24m (EM) $ 365.00
ET535/30m (EM) $ 365.00
ET632/60m (EM) $ 365.00
$2,200.00
Discounted Options
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF69904-EM

ET - 442/514/561nm Laser Triple Band Set for TIRF applications

For use with lasers between 437-448nm, 511-516nm and 559-564nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET442/514/561x (EX) $ 550.00
ZT442/514/561rpc (BS) $ 650.00
ET480/40m (EM) $ 365.00
ET540/30m (EM) $ 365.00
ET575lp (EM) $ 365.00
ZET442/514/561m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $3,100.00
Mounted in a Laser TIRF for Olympus BX2 models $3,225.00
Mounted in a Laser TIRF for Zeiss Axio $2,975.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $3,225.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59902-EM

ET – 442/514nm Laser Dual Band Set for TIRF applications

For use with lasers between 439-445nm and 512-517nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET442/514x (EX) $ 500.00
ZT442/514rpc (BS) $ 625.00
ET480/40m (EM) $ 365.00
ET545/40m (EM) $ 365.00
ZET442/514m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,650.00
Mounted in a Laser TIRF for Olympus BX2 models $2,775.00
Mounted in a Laser TIRF for Zeiss Axio $2,525.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,775.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF69905-EM

ET - 442/514/594nm Laser Triple Band Set for TIRF applications

For use with lasers between 400-449nm, 511-518nm and 588-597 nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET445/514/594x (EX) $ 550.00
ZT445/514/594rpc (BS) $ 650.00
ET480/40m (EM) $ 365.00
ET550/50m (EM) $ 365.00
ET645/75m (EM) $ 365.00
ZET445/514/594m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $3,100.00
Mounted in a Laser TIRF for Olympus BX2 models $3,225.00
Mounted in a Laser TIRF for Zeiss Axio $2,975.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $3,225.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59903-EM

ET – 442/532nm Laser Dual Band Set for TIRF applications

For use with lasers between 438-445nm and 530-535nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET442/532x (EX) $ 500.00
ZT442/532rpc (BS) $ 625.00
ET500/40m (EM) $ 365.00
ET585/65m (EM) $ 365.00
ZET442/532m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,650.00
Mounted in a Laser TIRF for Olympus BX2 models $2,775.00
Mounted in a Laser TIRF for Zeiss Axio $2,525.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,775.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  Filters in this Set   Price
ET470/40x (EX) $ 365.00
ET545/25x (EX) $ 365.00
ET640/30x (EX) $ 365.00
89016bs (BS) $ 425.00
ET520/40m (EM) $ 365.00
ET595/50m (EM) $ 365.00
ET690/50m (EM) $ 365.00
$2,220.00
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
  • Overview
  Filters in this Set   Price
ET470/40x (EX) $ 365.00
ET545/25x (EX) $ 365.00
59004bs (BS) $ 375.00
ET520/40m (EM) $ 365.00
ET605/70m (EM) $ 365.00
$1,600.00
Discounted Options
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59908-EM

ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation

For use with imaging lasers between 486-492nm and 558-563nm and photoactivation laser 400-410nm

Set includes the same filter combination as the 59908 PALM set, but with the 2mm TIRF dichroic.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET405/488/561x (EX) $ 550.00
ZT405/488/561rpc (BS) $ 650.00
ET525/50m (EM) $ 365.00
ET575lp (EM) $ 365.00
ZET488/561m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,750.00
Mounted in a Laser TIRF for Olympus BX2 models $2,875.00
Mounted in a Laser TIRF for Zeiss Axio $2,625.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,875.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59904-EM

ET – 488/561nm Laser Dual Band Set for TIRF applications

For use with lasers between 486-492nm and 556-563nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET488/561x (EX) $ 500.00
ZT488/561rpc (BS) $ 625.00
ET525/50m (EM) $ 365.00
ET610/75m (EM) $ 365.00
ZET488/561m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,650.00
Mounted in a Laser TIRF for Olympus BX2 models $2,775.00
Mounted in a Laser TIRF for Zeiss Axio $2,525.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,775.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FRET
  • ET Series
  • White Light

89021

ET - EGFP/mCherry

Best with these fluorochromes

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET470/40x (EX) $ 365.00
ET572/35x (EX) $ 365.00
59022bs (BS) $ 375.00
ET525/50m (EM) $ 365.00
ET632/60m (EM) $ 365.00
$1,600.00
Discounted Options
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59905-EM

ET – 488/594nm Laser Dual Band Set for TIRF applications

For use with lasers between 486-492nm and 591-596nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET488/594x (EX) $ 500.00
ZT488/594rpc (BS) $ 625.00
ET525/50m (EM) $ 365.00
ET645/75m (EM) $ 365.00
ZET488/594m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,650.00
Mounted in a Laser TIRF for Olympus BX2 models $2,775.00
Mounted in a Laser TIRF for Zeiss Axio $2,525.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,775.00
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59906-EM

ET – 488/640nm Laser Dual Band Set for TIRF applications

For use with lasers between 486-492nm and 633-647nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET488/640x (EX) $ 500.00
ZT488/640rpc (BS) $ 625.00
ET525/50m (EM) $ 365.00
ET655lp (EM) $ 365.00
ZET488/640m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,650.00
Mounted in a Laser TIRF for Olympus BX2 models $2,775.00
Mounted in a Laser TIRF for Zeiss Axio $2,525.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,775.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FRET
  • ET Series
  • White Light

89017

ET - FITC/Texas Red®

Best with these fluorochromes

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET490/20x (EX) $ 365.00
ET572/35x (EX) $ 365.00
69002bs (BS) $ 425.00
ET535/50m (EM) $ 365.00
ET632/60m (EM) $ 365.00
$1,600.00
Discounted Options
Mounting options are available when adding to cart.
  • Show:
  • Transmission
  • Optical Density
 
  • Fluorescence
  • TIRF
  • FRET
  • ET Series
  • Laser and laser diodes

TRF59907-EM

ET – 532/640nm Laser Dual Band Set for TIRF applications

For use with lasers between 528-535nm and 633-647nm

Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.

Coating: Sputter/Hard Coated

Dichroic beamsplitters for all TIRF filter cubes are 2mm thick

  Filters in this Set   Price
ZET532/640x (EX) $ 500.00
ZT532/640rpc (BS) $ 625.00
ET585/65m (EM) $ 365.00
ET655lp (EM) $ 365.00
ZET532/640m (EM) $ 575.00
Mounted in a Laser TIRF for Nikon TE2000/Ti $2,650.00
Mounted in a Laser TIRF for Olympus BX2 models $2,775.00
Mounted in a Laser TIRF for Zeiss Axio $2,525.00
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters $2,775.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FRET
  • ET Series
  • White Light

89022

ET - Cy3/Cy5

Best with these fluorochromes

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET545/25x (EX) $ 365.00
ET640/30x (EX) $ 365.00
59007bs (BS) $ 375.00
ET595/50m (EM) $ 365.00
ET690/50m (EM) $ 365.00
$1,550.00
Discounted Options
Mounting options are available when adding to cart.
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