FRET
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
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Showing 24 products
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Product Type
Fluorochrome
- Acridine Orange + DNA (15) Apply Acridine Orange + DNA filter
- Alexa Fluor 350™ (7) Apply Alexa Fluor 350™ filter
- Alexa Fluor 405 (7) Apply Alexa Fluor 405 filter
- Alexa Fluor 488™ (15) Apply Alexa Fluor 488™ filter
- Alexa Fluor 514™ (6) Apply Alexa Fluor 514™ filter
- Alexa Fluor 532™ (2) Apply Alexa Fluor 532™ filter
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- Allophycocyanin (APC) (6) Apply Allophycocyanin (APC) filter
- AsRed2 (18) Apply AsRed2 filter
- Atto 425 (7) Apply Atto 425 filter
- Atto 488 (15) Apply Atto 488 filter
- Atto 550 (11) Apply Atto 550 filter
- Atto 647N (6) Apply Atto 647N filter
- Azami Green (15) Apply Azami Green filter
- BCECF/pH 9.0 (15) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (15) Apply BODIPY FL/pH7.2 filter
- Biosearch Blue (7) Apply Biosearch Blue filter
- Brilliant Violet™ 421 (7) Apply Brilliant Violet™ 421 filter
- Brilliant Violet™ 480 (7) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Gold 540 (2) Apply CAL Fluor® Gold 540 filter
- CAL Fluor® Orange 560 (2) Apply CAL Fluor® Orange 560 filter
- CAL Fluor® Red 590 (18) Apply CAL Fluor® Red 590 filter
- CAL Fluor® Red 610 (7) Apply CAL Fluor® Red 610 filter
- CFP (7) Apply CFP filter
- Calcein (15) Apply Calcein filter
- Calcium Green™-1 (6) Apply Calcium Green™-1 filter
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- Cy3™ (11) Apply Cy3™ filter
- Cy5™ (6) Apply Cy5™ filter
- DAPI (7) Apply DAPI filter
- DiD (6) Apply DiD filter
- DiI (11) Apply DiI filter
- DiO (15) Apply DiO filter
- Draq5 (6) Apply Draq5 filter
- DsRed (11) Apply DsRed filter
- DyLight 350 (7) Apply DyLight 350 filter
- DyLight 405 (7) Apply DyLight 405 filter
- DyLight 488 (15) Apply DyLight 488 filter
- DyLight 549 (11) Apply DyLight 549 filter
- DyLight 594 (7) Apply DyLight 594 filter
- DyLight 649 (6) Apply DyLight 649 filter
- EBFP2 (7) Apply EBFP2 filter
- ECFP (7) Apply ECFP filter
- EGFP (15) Apply EGFP filter
- EYFP/pH 7 (6) Apply EYFP/pH 7 filter
- Emerald GFP (15) Apply Emerald GFP filter
- Eosin (2) Apply Eosin filter
- FAM (15) Apply FAM filter
- FITC (15) Apply FITC filter
- FM™ 1-43 (15) Apply FM™ 1-43 filter
- FM™ 4-64 (6) Apply FM™ 4-64 filter
- FlAsH-CCPFCC (6) Apply FlAsH-CCPFCC filter
- Fluo-3 (21) Apply Fluo-3 filter
- Fluo-4 (15) Apply Fluo-4 filter
- FusionRed (18) Apply FusionRed filter
- GFP (15) Apply GFP filter
- HEX,SE (2) Apply HEX,SE filter
- Hoechst 33258 (7) Apply Hoechst 33258 filter
- Killer Red (7) Apply Killer Red filter
- Lucifer Yellow (7) Apply Lucifer Yellow filter
- LysoTracker Blue/MeOH (7) Apply LysoTracker Blue/MeOH filter
- LysoTracker Green/pH 5.2 (15) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (18) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (15) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Deep Red 633/MeOH (6) Apply MitoTracker Deep Red 633/MeOH filter
- MitoTracker Green FM/MeOH (15) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Orange/MeOH (11) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (18) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (15) Apply Oregon Green™ 488 filter
- Oregon Green™ 514 (6) Apply Oregon Green™ 514 filter
- Pacific Blue (7) Apply Pacific Blue filter
- Propidium Iodide (13) Apply Propidium Iodide filter
- Qdot 525 (7) Apply Qdot 525 filter
- Qdot 585 (4) Apply Qdot 585 filter
- Qdot 605 (6) Apply Qdot 605 filter
- Qdot 705 (2) Apply Qdot 705 filter
- Quasar® 570 (11) Apply Quasar® 570 filter
- Quasar® 670 (6) Apply Quasar® 670 filter
- R-phycoerythrin (11) Apply R-phycoerythrin filter
- ROX (18) Apply ROX filter
- ReAsH-CCPGCC (7) Apply ReAsH-CCPGCC filter
- Resorufin (7) Apply Resorufin filter
- Rhod-2 (11) Apply Rhod-2 filter
- Rhodamine 6G (2) Apply Rhodamine 6G filter
- Rhodamine 123 (6) Apply Rhodamine 123 filter
- Rhodamine Red™-X (18) Apply Rhodamine Red™-X filter
- SNARF pH 9.0 (7) Apply SNARF pH 9.0 filter
- SYBR® Green I (15) Apply SYBR® Green I filter
- SYTO 9/DNA (15) Apply SYTO 9/DNA filter
- SYTO® 60 (6) Apply SYTO® 60 filter
- Sulforhodamine 101 (7) Apply Sulforhodamine 101 filter
- T-Sapphire (7) Apply T-Sapphire filter
- TAMRA (11) Apply TAMRA filter
- TO-PRO™-3 (6) Apply TO-PRO™-3 filter
- TRITC (11) Apply TRITC filter
- TagBFP (7) Apply TagBFP filter
- TagRFP (11) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (11) Apply Tetramethylrhodamine isothiocyanate filter
- Texas Red® (7) Apply Texas Red® filter
- Texas Red®-X (7) Apply Texas Red®-X filter
- Topaz (6) Apply Topaz filter
- TurboFP650 (7) Apply TurboFP650 filter
- X-rhod-1/Ca2+ (18) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (15) Apply ZsGreen1 filter
- ZsYellow (2) Apply ZsYellow filter
- mCherry (7) Apply mCherry filter
- mCitrine (6) Apply mCitrine filter
- mKO (2) Apply mKO filter
- mKate2 (7) Apply mKate2 filter
- mKeima Red (7) Apply mKeima Red filter
- mOrange2 (2) Apply mOrange2 filter
- mPlum (7) Apply mPlum filter
- mRFP1 (7) Apply mRFP1 filter
- mWasabi (15) Apply mWasabi filter
- tdTomato (11) Apply tdTomato filter
Application
Center/Edge/Notch Wavelength
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TRF59901-EM
ET – 405/488nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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TRF69901-EM
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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TRF89902-EM
ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
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TRF69902-EM
ET - 405/488/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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89013
ET - DAPI/FITC/TRITC
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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89000
ET - Sedat Quad
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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- Transmission
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- Overview
89014
ET - DAPI/FITC/Texas Red®
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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- Transmission
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- Overview
89002
ET - ECFP/EYFP
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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- Transmission
- Optical Density
- Overview
89007
ET - ECFP/EYFP/DsRed
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
89006
ET - ECFP/EYFP/mCherry
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
TRF59902-EM
ET – 442/514nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
TRF69905-EM
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59903-EM
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
- Overview
89016
ET - FITC/Cy3/Cy5
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
89019
ET - FITC/TRITC
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
TRF59908-EM
ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
Set includes the same filter combination as the 59908 PALM set, but with the 2mm TIRF dichroic.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
TRF59904-EM
ET – 488/561nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
- Overview
89021
ET - EGFP/mCherry
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
TRF59905-EM
ET – 488/594nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59906-EM
ET – 488/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
- Overview
89017
ET - FITC/Texas Red®
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
TRF59907-EM
ET – 532/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
- Overview
89022
ET - Cy3/Cy5
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
