Ratiometric
Ratiometric imaging uses a ratio of images to determine ion concentration, voltage or pH levels in a cell of a cell network. One common example is the Fura-2 fluorophore, which absorbs light preferentially at 380nm in high intracellular concentrations of calcium and absorbs light preferentially at 340nm in low concentrations of calcium. By exciting the sample at these two wavelengths and taking a ratio of the image, a semi-quantitative determination of calcium levels is possible. Other ratiometric fluorophores, like INDO-1, undergo a change to their emission wavelength under differing ion concentrations.
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Ratiometric Calcium Indicators - Fluorescence Excitation Spectra of Fura2
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Showing 8 products
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Product Type
Fluorochrome
- BCECF/pH 5.2 (1) Apply BCECF/pH 5.2 filter
- BCECF/pH 9.0 (1) Apply BCECF/pH 9.0 filter
- Fura-2/Ca2+ - free (1) Apply Fura-2/Ca2+ - free filter
- Fura-2/Ca2+ - saturated (1) Apply Fura-2/Ca2+ - saturated filter
- Indo-1/Ca2+ -free (1) Apply Indo-1/Ca2+ -free filter
- Indo-1/Ca2+ -saturated (1) Apply Indo-1/Ca2+ -saturated filter
- SBFI/Na+ -free (1) Apply SBFI/Na+ -free filter
- SBFI/Na+ -saturated (1) Apply SBFI/Na+ -saturated filter
Application
Center/Edge/Notch Wavelength
Emission passbands
- Show:
- Transmission
- Optical Density
- Overview
79006
ET - INDO
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
79003
ET - Fura 2/TRITC
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
79001
ET - Fura 2
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
79004
ET - Fura 2/GFP
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
79005
ET - BCECF
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
79002
ET - Fura 2/BCECF
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
79009
ET - SNARF with 500nm excitation
This filter set is intended for dual-emission ratiometric measurements of the SNARF family of pH indicators when excited with light in the 490-510nm wavelength range.
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
79010
ET - SNARF with 550nm excitation
This filter set is intended for dual-emission ratiometric measurements of the SNARF family of pH indicators when excited with light in the 540-560nm wavelength range.
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
