TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
Showing 39 products
- (-) Remove TIRF filter TIRF
- (-) Remove Catalog Sets filter Catalog Sets
Product Type
Fluorochrome
- Acridine Orange + DNA (15) Apply Acridine Orange + DNA filter
- Alexa Fluor 350™ (8) Apply Alexa Fluor 350™ filter
- Alexa Fluor 405 (8) Apply Alexa Fluor 405 filter
- Alexa Fluor 488™ (15) Apply Alexa Fluor 488™ filter
- Alexa Fluor 514™ (6) Apply Alexa Fluor 514™ filter
- Alexa Fluor 532™ (4) Apply Alexa Fluor 532™ filter
- Alexa Fluor 546™ (9) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (9) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (15) Apply Alexa Fluor 568™ filter
- Alexa Fluor 594™ (6) Apply Alexa Fluor 594™ filter
- Alexa Fluor 647™ (6) Apply Alexa Fluor 647™ filter
- Allophycocyanin (APC) (6) Apply Allophycocyanin (APC) filter
- AsRed2 (15) Apply AsRed2 filter
- Atto 425 (8) Apply Atto 425 filter
- Atto 488 (15) Apply Atto 488 filter
- Atto 550 (9) Apply Atto 550 filter
- Atto 647N (6) Apply Atto 647N filter
- Azami Green (15) Apply Azami Green filter
- BCECF/pH 9.0 (15) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (15) Apply BODIPY FL/pH7.2 filter
- Biosearch Blue (8) Apply Biosearch Blue filter
- Brilliant Violet™ 421 (8) Apply Brilliant Violet™ 421 filter
- Brilliant Violet™ 480 (8) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Gold 540 (4) Apply CAL Fluor® Gold 540 filter
- CAL Fluor® Orange 560 (4) Apply CAL Fluor® Orange 560 filter
- CAL Fluor® Red 590 (15) Apply CAL Fluor® Red 590 filter
- CAL Fluor® Red 610 (6) Apply CAL Fluor® Red 610 filter
- CFP (8) Apply CFP filter
- Calcein (15) Apply Calcein filter
- Calcium Green™-1 (6) Apply Calcium Green™-1 filter
- Cerulean (8) Apply Cerulean filter
- Citrine (6) Apply Citrine filter
- Cy2™ (15) Apply Cy2™ filter
- Cy3.5™ (15) Apply Cy3.5™ filter
- Cy3™ (9) Apply Cy3™ filter
- Cy5™ (6) Apply Cy5™ filter
- DAPI (8) Apply DAPI filter
- DiD (6) Apply DiD filter
- DiI (9) Apply DiI filter
- DiO (15) Apply DiO filter
- Draq5 (6) Apply Draq5 filter
- DsRed (9) Apply DsRed filter
- DyLight 350 (8) Apply DyLight 350 filter
- DyLight 405 (8) Apply DyLight 405 filter
- DyLight 488 (15) Apply DyLight 488 filter
- DyLight 549 (9) Apply DyLight 549 filter
- DyLight 594 (6) Apply DyLight 594 filter
- DyLight 649 (6) Apply DyLight 649 filter
- EBFP2 (8) Apply EBFP2 filter
- ECFP (8) Apply ECFP filter
- EGFP (15) Apply EGFP filter
- EYFP/pH 7 (6) Apply EYFP/pH 7 filter
- Emerald GFP (15) Apply Emerald GFP filter
- Eosin (4) Apply Eosin filter
- FAM (15) Apply FAM filter
- FITC (15) Apply FITC filter
- FM™ 1-43 (15) Apply FM™ 1-43 filter
- FM™ 4-64 (6) Apply FM™ 4-64 filter
- FlAsH-CCPFCC (6) Apply FlAsH-CCPFCC filter
- Fluo-3 (21) Apply Fluo-3 filter
- Fluo-4 (15) Apply Fluo-4 filter
- FusionRed (15) Apply FusionRed filter
- GFP (15) Apply GFP filter
- HEX,SE (4) Apply HEX,SE filter
- Hoechst 33258 (8) Apply Hoechst 33258 filter
- Killer Red (6) Apply Killer Red filter
- Lucifer Yellow (8) Apply Lucifer Yellow filter
- LysoTracker Blue/MeOH (8) Apply LysoTracker Blue/MeOH filter
- LysoTracker Green/pH 5.2 (15) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (15) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (15) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Deep Red 633/MeOH (6) Apply MitoTracker Deep Red 633/MeOH filter
- MitoTracker Green FM/MeOH (15) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Orange/MeOH (9) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (15) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (15) Apply Oregon Green™ 488 filter
- Oregon Green™ 514 (6) Apply Oregon Green™ 514 filter
- Pacific Blue (8) Apply Pacific Blue filter
- Propidium Iodide (13) Apply Propidium Iodide filter
- Qdot 525 (8) Apply Qdot 525 filter
- Qdot 585 (4) Apply Qdot 585 filter
- Qdot 605 (6) Apply Qdot 605 filter
- Qdot 705 (2) Apply Qdot 705 filter
- Quasar® 570 (9) Apply Quasar® 570 filter
- Quasar® 670 (6) Apply Quasar® 670 filter
- R-phycoerythrin (9) Apply R-phycoerythrin filter
- ROX (15) Apply ROX filter
- ReAsH-CCPGCC (6) Apply ReAsH-CCPGCC filter
- Resorufin (6) Apply Resorufin filter
- Rhod-2 (9) Apply Rhod-2 filter
- Rhodamine 6G (4) Apply Rhodamine 6G filter
- Rhodamine 123 (6) Apply Rhodamine 123 filter
- Rhodamine Red™-X (15) Apply Rhodamine Red™-X filter
- SNARF pH 9.0 (6) Apply SNARF pH 9.0 filter
- SYBR® Green I (15) Apply SYBR® Green I filter
- SYTO 9/DNA (15) Apply SYTO 9/DNA filter
- SYTO® 60 (6) Apply SYTO® 60 filter
- Sulforhodamine 101 (6) Apply Sulforhodamine 101 filter
- T-Sapphire (8) Apply T-Sapphire filter
- TAMRA (9) Apply TAMRA filter
- TO-PRO™-3 (6) Apply TO-PRO™-3 filter
- TRITC (9) Apply TRITC filter
- TagBFP (8) Apply TagBFP filter
- TagRFP (9) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (9) Apply Tetramethylrhodamine isothiocyanate filter
- Texas Red® (6) Apply Texas Red® filter
- Texas Red®-X (6) Apply Texas Red®-X filter
- Topaz (6) Apply Topaz filter
- TurboFP650 (6) Apply TurboFP650 filter
- X-rhod-1/Ca2+ (15) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (15) Apply ZsGreen1 filter
- ZsYellow (4) Apply ZsYellow filter
- mCherry (6) Apply mCherry filter
- mCitrine (6) Apply mCitrine filter
- mKO (4) Apply mKO filter
- mKate2 (6) Apply mKate2 filter
- mKeima Red (8) Apply mKeima Red filter
- mOrange2 (4) Apply mOrange2 filter
- mPlum (6) Apply mPlum filter
- mRFP1 (6) Apply mRFP1 filter
- mWasabi (15) Apply mWasabi filter
- tdTomato (9) Apply tdTomato filter
Application
Center/Edge/Notch Wavelength
- Show:
- Transmission
- Optical Density
TRF49901
ET - 405nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89901-EMv2
ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89901v2
ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
Set includes no additional emission filters; instead, the quad band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69901
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59901
ET – 405/488nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69902
ET - 405/488/594nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59901-EM
ET – 405/488nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69901-EM
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89902
ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
Set includes no additional emission filters; instead, the quad band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89902-EM
ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69902-EM
ET - 405/488/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF49902
ET - 440nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF49903
ET - 440nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59902
ET – 442/514nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69904
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59902-EM
ET – 442/514nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69905
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69905-EM
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59903
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59903-EM
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF49904
ET - 488nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59904
ET – 488/561nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59908-EM
ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
Set includes the same filter combination as the 59908 PALM set, but with the 2mm TIRF dichroic.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59904-EM
ET – 488/561nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
