Guides & Instructions

A practical guide to fluorescence and optical filters

Best practices for safely handling, cleaning, and storing Chroma filters to protect coatings and maintain long-term performance.

How to identify filter surfaces and orient filters and dichroics correctly for best performance.

Short, focused resources explaining how to apply optical filters effectively in real-world imaging, detection, and measurement systems.

Optical cubes serve as conductors guiding excitation and emission light through precisely arranged filters and mirrors. Correct assembly ensures accurate spectral performance.

Definitions of common optical terms used across fluorescence, imaging, and thin-film optics.

A comprehensive handbook on optical filters for fluorescence microscopy, including design principles, filter terminology, and system-level considerations.

Printable pamphlet with the basics of optical filters and their applications.

Pinholes are tiny defects that can form in the coating process. While they may be small, their effects can be significant, leading to issues such as light leakage or reduced durability.

Learn how different fluorescence filter configurations, including Pinkel, Sedat, and others, can balance imaging speed and fluorophore specificity in high-speed multicolor live-cell microscopy systems.

Learn how to add filters, customize plots, export data, and more.

When designing a fluorescence or analytical optical system, the dichroic beamsplitter plays a central role. Learn more about our ZT and T Dichroic Mirrors.