Nikon TE2000/Ti Assembly Instructions
This guide provides step-by-step instructions for assembling a Nikon TE2000/Ti model (Chroma part number 91020), designed for precise alignment of optical filters in fluorescence microscopy systems. Proper assembly ensures optimal performance and system reliability.
For those new to handling optical filters, we recommend reviewing our Cleaning & Handling Instructions before assembling your filter cube.
Need a printable version?
Download the cube assembly instructions as an easy-to-use reference for the lab.
Nikon TE2000/Ti
Required filter sizes
Exciter (x) | Ø25mm |
Emitter (m) | Ø25mm |
Dichroic | 25.5 x 36mm |
Required tools
You don't need anything. It's push-and-click for the beamsplitter, and the retaining rings are screwed in by hand (the emitter ring requires a delicate touch to find the sweet spot needed to properly thread the ring).
Instructions
Step 1
Disassemble the cube. You should have the exciter cap and reversible retaining ring, the emitter mount and retaining ring, and the clip and spring for the beam splitter.
Step 2
Gently place the beam splitter down into the cube at an angle with the arrow on the side of the filter pointing down.
Place the spring gently on top of the beam splitter. Push the clip on top of the spring and push until you hear a click.
Step 3
Place the exciter into the exciter port in front of the cube with the arrow facing in towards the beam splitter.
Step 4
A note for mounting the exciter – depending on the thickness of the filter, the retaining ring can be reversed. For thicker filters, insert the ring into the cap with the wider side facing towards it (as seen in the photo). For thing filters, reverse the ring.
Step 5
The emitter goes into the emitter port on the top of the cube, or the piece currently separated from the cube, with the arrow pointing down.
Screw in the retaining ring to secure the filter.
Step 6
The emitter hold is then placed back on the cube. The end tabs go in first, aligning with the notches in the cube. Once the front is in, push the back end down until it clicks.
Final Checks
Before installing the assembled cube into your optical system, confirm the following:
Filter Orientation
Verify that all filter arrows are aligned correctly. The beamsplitter arrow should face upward. The exciter and emitter filter arrows should point inward, toward the beam splitter.
Beamsplitter Alignment
Check that the registration tabs and holes between the cube halves are fully engaged. The two halves should meet cleanly, with no visible gaps or misalignment.
Filter Seating
Ensure that the excitation and emission filters are properly installed. Filters should be fully inserted into their respective ports. Retaining rings should be threaded evenly to hold the filters securely, without tilt or cross-threading.
Mechanical Stability
Confirm that the screws joining the cube halves are tightened. The cube should feel stable when handled, with no internal movement or looseness.
Visual Inspection
Inspect all optical surfaces for contamination and use cleanroom practices when handling filters. Refer to Chroma’s Cleaning and Handling Guidelines if cleaning is required.
If you encounter alignment issues, looseness, or other concerns during this inspection, disassemble and repeat the relevant steps. For additional support, please contact Chroma Technical Support.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet your specifications.
