Leica P Cubes Assembly Instructions
This guide provides step-by-step instructions for assembling a Leica P filter cube (Chroma part numbers 91050 and 91051), designed for precise alignment of optical filters in fluorescence microscopy systems. Proper assembly ensures optimal performance and system reliability.
For those new to handling optical filters, we recommend reviewing our Cleaning & Handling Instructions before assembling your filter cube.
Need a printable version?
Download the cube assembly instructions as an easy-to-use reference for the lab.
Leica P Filter Cube
Required filter sizes
Exciter (x) | Ø25mm |
Emitter (m) | Ø25mm |
Dichroic | 25.5 x 36mm 1mm to 3mm thick |
Required tools
2mm hex driver
Instructions
Step 1
Remove the four small screws holding the cube together, two inset on the top and two inset on the front. Each screw has a small washer as well.
Step 2
Remove the two long screws on cube in the photo to separate the cube halves.
Step 3
Place the cube half with the beam splitter frame on a stable surface to keep the cube level.
Step 4
Place the beam splitter in the cube with the arrow on the side of the filter point up.
Step 5
Rest the other half the cube on top of the half with the beam splitter, aligning the screw holes. Once aligned, use the hex driver to mount the two longer screws.
Step 6
The exciter goes in the exciter port on the front of the cube with the arrow pointing in towards the beam splitter.
Secure the exciter with two screws.
Step 7
The emitter goes into the emitter port on the top of the cube with the arrow pointing in towards the beam splitter.
Secure the filter with the two remaining screws.
Step 8
Fully assembled cube
Final Checks
Before installing the assembled cube into your optical system, confirm the following:
Filter Orientation
Verify that all filter arrows are aligned correctly. The beamsplitter arrow should face upward. The exciter and emitter filter arrows should point inward, toward the beam splitter.
Beamsplitter Alignment
Check that the registration tabs and holes between the cube halves are fully engaged. The two halves should meet cleanly, with no visible gaps or misalignment.
Filter Seating
Ensure that the excitation and emission filters are properly installed. Filters should be fully inserted into their respective ports. Retaining rings should be threaded evenly to hold the filters securely, without tilt or cross-threading.
Mechanical Stability
Confirm that the screws joining the cube halves are tightened. The cube should feel stable when handled, with no internal movement or looseness.
Visual Inspection
Inspect all optical surfaces for contamination and use cleanroom practices when handling filters. Refer to Chroma’s Cleaning and Handling Guidelines if cleaning is required.
If you encounter alignment issues, looseness, or other concerns during this inspection, disassemble and repeat the relevant steps. For additional support, please contact Chroma Technical Support.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet your specifications.
