Zeiss Axio Cube Assembly Instructions
This guide provides step-by-step instructions for assembling a Zeiss Axio filter cube (Chroma part number 91029), designed for precise alignment of optical filters in fluorescence microscopy systems. Proper assembly ensures optimal performance and system reliability.
For those new to handling optical filters, we recommend reviewing our Cleaning & Handling Instructions before assembling your filter cube.
Required filter sizes
Exciter (x) | Ø25mm |
Emitter (m) | Ø25mm |
Beamsplitter | 25.5 × 36mm |
Required tools
Spanner tool to tighten the retaining rings. Note: If you don’t have this tool, you can use tweezers once the ring has been seated, but be careful not to slip and mar the filter.
Need a printable version?
Download the cube assembly instructions as an easy-to-use reference for the lab.
Step 1
Remove the retaining rings and separate the cube halves.
Step 2
Place the half with the springs on a surface to keep the cube level.
Step 3
Place the beam splitter on the cube half so it rests on the spring, with the arrow facing up. The arrow can be found on the side of the filter.
Step 4
Align the registration tabs and holes on both filters. Once tabs are aligned, move the cube half on top of the beam splitter so that the bottom holes slide over the bottom tabs. Once the halves are together, secure the cube together with screws.
Step 5
Place the exciter filter in the exciter port with the arrow facing in, towards the beam splitter.
Step 6
Screw the retaining ring in to secure the filter.
Final Checks
Before installing the assembled cube into your optical system, confirm the following:
Filter Orientation
Verify that all filter arrows are aligned correctly. The beamsplitter arrow should face upward. The exciter and emitter filter arrows should point inward, toward the beam splitter.
Beamsplitter Alignment
Check that the registration tabs and holes between the cube halves are fully engaged. The two halves should meet cleanly, with no visible gaps or misalignment.
Filter Seating
Ensure that the excitation and emission filters are properly installed. Filters should be fully inserted into their respective ports. Retaining rings should be threaded evenly to hold the filters securely, without tilt or cross-threading.
Mechanical Stability
Confirm that the screws joining the cube halves are tightened. The cube should feel stable when handled, with no internal movement or looseness.
Visual Inspection
Inspect all optical surfaces for contamination and use cleanroom practices when handling filters. Refer to Chroma’s Cleaning and Handling Guidelines if cleaning is required.
If you encounter alignment issues, looseness, or other concerns during this inspection, disassemble and repeat the relevant steps. For additional support, please contact Chroma Technical Support.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet your specifications.
