Application Guides and Notes
Technical resources covering filter selection across fluorescence microscopy techniques and other applications.
Fluorescence Microscopy
Confocal Microscopy
Laser clean-up filters, polychroics, and emission blocking for scanning confocal
FRET Microscopy
How to choose excitation and emission filters for maximum donor-acceptor signal separation in FRET experiments
Introduction to FRET Application Note
Printable reference for FRET filter selection and donor/acceptor separation
Multiphoton Microscopy
Dichroic requirements, NIR blocking, and barrier filters for 2p and 3p systems
Quantum Dots (Qdots)
Shortpass excitation and narrow bandpass emission strategies for Qdot detection
Ratiometry
Filter selection for ratiometric dyes like Fura-2, including excitation switching and emission separation approaches
TIRF Microscopy
OD 8+ blocking and dichroic flatness for TIRF microscopy
Intro to TIRF Application Note
Covers prism-based and through-the-lens TIRF configurations, with specific filter set examples
5-channel Fluorescence Imaging Simplified
Multiband filter set design for five-color fluorescence imaging
Fluorescence Filters for FISH
Filter set design for fluorescence in situ hybridization
Spectroscopy & Laser Systems
Raman Spectroscopy
Suppressing Rayleigh scatter and selecting laser clean-up filters for Raman systems
Coherent Anti-Stokes Raman Scattering
Filter requirements for CARS microscopy
Filters and Mirrors for Laser Applications
Technical reference for filter and dichroic selection across common laser-based imaging techniques
Other Applications
Flow Cytometry
AOI considerations, PMT detection, and multicolor filter selection for flow systems
Plate Readers
Excitation and emission filter selection for bulk fluorescence and absorbance assays
Multispectral Imaging in the SWIR
Short-wave infrared filter selection for multispectral imaging systems
Colorimetry, Photometry & Light Balancing
Sputtered coating design and broadband monitoring for CIE tristimulus and light balance filters, with f1' results
Sputtered Coatings for Space-Based Systems
Coating technology for satellite communications and aerospace optics
