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Application Guides and Notes

Technical resources covering filter selection across fluorescence microscopy techniques and other applications.

Fluorescence Microscopy

Confocal Microscopy

Laser clean-up filters, polychroics, and emission blocking for scanning confocal

FRET Microscopy

How to choose excitation and emission filters for maximum donor-acceptor signal separation in FRET experiments

Introduction to FRET Application Note

Printable reference for FRET filter selection and donor/acceptor separation

Multiphoton Microscopy

Dichroic requirements, NIR blocking, and barrier filters for 2p and 3p systems

Quantum Dots (Qdots)

Shortpass excitation and narrow bandpass emission strategies for Qdot detection

Ratiometry

Filter selection for ratiometric dyes like Fura-2, including excitation switching and emission separation approaches

TIRF Microscopy

OD 8+ blocking and dichroic flatness for TIRF microscopy

Intro to TIRF Application Note

Covers prism-based and through-the-lens TIRF configurations, with specific filter set examples

5-channel Fluorescence Imaging Simplified

Multiband filter set design for five-color fluorescence imaging

Fluorescence Filters for FISH

Filter set design for fluorescence in situ hybridization

Spectroscopy & Laser Systems

Raman Spectroscopy

Suppressing Rayleigh scatter and selecting laser clean-up filters for Raman systems

Coherent Anti-Stokes Raman Scattering

Filter requirements for CARS microscopy

Filters and Mirrors for Laser Applications

Technical reference for filter and dichroic selection across common laser-based imaging techniques

Other Applications

Flow Cytometry

AOI considerations, PMT detection, and multicolor filter selection for flow systems

Plate Readers

Excitation and emission filter selection for bulk fluorescence and absorbance assays

Multispectral Imaging in the SWIR

Short-wave infrared filter selection for multispectral imaging systems

Colorimetry, Photometry & Light Balancing

Sputtered coating design and broadband monitoring for CIE tristimulus and light balance filters, with f1' results

Sputtered Coatings for Space-Based Systems

Coating technology for satellite communications and aerospace optics

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